丁酸钠联合顺铂对卵巢透明细胞癌ES-2细胞活性及增殖的影响

Effect of sodium butyrate combined with cisplatin on cell viability and proliferation of ovarian clear cell carcinoma ES-2 cells

目的观察丁酸钠联合顺铂对卵巢透明细胞癌ES-2细胞活性及增殖的影响。方法将对数生长期的卵巢透明细胞癌ES-2细胞随机分为观察组和对照组。观察组分别加入1、2、4、6 mmol/L的丁酸钠和5μg/m L顺铂,对照组分别加入与观察组相同浓度的丁酸钠。采用MMT法检测两组细胞活性,Western blotting法检测两组凋亡蛋白(c-PAPP、c-Caspase-3)和增殖蛋白(ERK、p-ERK)相对表达量。结果观察组加入1、2、4、6 mmol/L丁酸钠和顺铂细胞活性分别为51%±3%、39%±2%、22%±2%、11%±1%,多组间及组内两两比较,P均<0.05;对照组加入1、2、4、6 mmol/L丁酸钠细胞活性分别为88%±4%、76%±3%、69%±2%、54%±2%,多组间及组内两两比较,P均<0.05;两组加入相同浓度丁酸钠细胞活性比较,P均<0.05。随着丁酸钠浓度增加,两组凋亡蛋白cPAPP、c-Caspase-3相对表达量逐渐升高,增殖蛋白ERK、p-ERK相对表达量逐渐降低。与对照组同浓度丁酸钠比较,观察组1、2、4、6 mmol/L丁酸钠和顺铂c-PAPP、c-Caspase-3蛋白表达均升高,1、6 mmol/L丁酸钠和顺铂ERK、pERK蛋白表达均降低;两组比较,P均<0.05。结论丁酸钠联合顺铂可降低卵巢透明细胞癌ES-2细胞活性,抑制其增殖、促进其凋亡;丁酸钠浓度越高,上述作用越明显。

Objective To observe the effect of sodium butyrate combined with cisplatin on cell viability and proliferation of ovarian clear cell carcinoma ES-2 cells. Methods The ovarian clear cell carcinoma ES-2 cells in the logarithmic phase were randomly divided into the observation groups and control groups. The observation groups were added 1,2,4and 6 mmol / L sodium butyrate( Na B) and 5 μg / m L cisplatin. The control groups were added the same concentrations of Na B. The cell activities were detected by MMT,and the relative expression levels of c-PAPP,c-Caspase-3,ERK and pERK were detected by Western blotting. Results The cell viabilities of the observation groups which were treated with 1,2,4,6 mmol / L Na B and 5 μg / m L cisplatin were 51% ± 3%,39% ± 2%,22% ± 2% and 11% ± 1%,respectively;and significant difference was found between groups( all P < 0. 05). The cell viabilities of the control groups were 88% ±4%,76% ± 3%,69% ± 2% and 54% ± 2%,respectively; and significant difference was found between groups( all P <0. 05). Meanwhile,significant difference was found in the cell viability between the two groups which were added with the same concentrations of Na B( all P < 0. 05). With the increased concentrations of Na B,the relative expression levels of cPAPP and c-Caspase-3 in the two kinds of groups were gradually increased,and the levels of ERK and p-ERK were significantly decreased. Compared with the control groups,the relative expression levels of c-PAPP and c-Caspase-3 in the observation groups which were added 1,2,4 and 6 mmol / L Na B and 5 μg / m L cisplatin were all increased and the levels of ERK and P-ERK were decreased in the observation groups which were added 1 and 6 mmol / L Na B and 5 μg / m L cisplatin.Significant difference was found between the two kinds of groups( all P < 0. 05). Conclusion Na B combined with cisplatin may decrease the cell viability of ovarian clear cell carcinoma ES-2 cells,inhibit the proliferation and promote the apoptosis. The effect can be stronger with the higher concentrations of Na B.