长链非编码RNAPVT1在肾癌组织中的表达及意义

Expression and clinical significance of lncRNA PTV1 in renal carcinoma tissues

目的探讨长链非编码RNA PTV1在肾癌组织的表达及意义。方法收集54例肾癌患者的癌组织,并选取距离肿瘤边缘2 cm以上(镜下未见癌细胞)的癌旁组织作为对照;提取总RNA、反转录获得c DNA后,采用Real-time PCR法检测肾癌及癌旁组织PTV1的相对表达量;分析肾癌组织PTV1的相对表达与患者临床病理特征及预后的关系。MTT方法检测肾癌细胞增殖水平、流式细胞术检测细胞凋亡率的改变。结果肾癌组织与癌旁组织PVT1的相对表达量分别为6.22±0.71和2.26±0.36,肾癌组织明显高于癌旁组织(P<0.05)。54份肾癌组织PVT1高表达39份,低表达15份,肾癌组织PTV1相对表达与患者淋巴结转移和TNM分期相关(P均<0.05),与性别、年龄及病理类型未见相关性(P均>0.05)。肾癌组织PVT1高表达与低表达者总体生存时间分别为(47.9±9.4)、(67.2±3.8)个月,PTV1高表达者的总体生存时间明显低于PTV1低表达者(P<0.05)。体外786-0肾癌细胞转染PVT1 siRNA,肾癌细胞增殖活性明显降低、凋亡率明显增高(P均<0.05)。结论 PVT1可能通过影响肾癌细胞增殖与凋亡促进肾癌的发生、发展,可作为潜在的肾癌治疗新靶点。

Objective To invesigate the expression of long non-coding RNA( lncRNA) PTV1 and its significance in the renal carcinoma tissues. Methods Fifty-four cases of renal cell carcinoma tissues were collected and the adjacent tissues of above 2 cm to tumor( no cancer cells were found under microscope) were used as the controls. Total RNA was isolated to get c DNA by performing reverse transcription. Then real-time quantitative PCR( RT-q PCR) was used to detect the expression of PTV1 in the renal cell carcinoma tissues and the adjacent tissues. Then the PTV1 expression level in the renal carcinoma tissues and the relationships between the expression of PTV1 and clinical characteristics and the prognosis were analyzed. The cell proliferation and apoptosis rate were tested by using MTT and flow cytometry,respectively. Results The relative expression levels of PVT1 in the renal carcinoma tissues and adjacent tissues were 6. 22 ± 0. 71 and 2. 26 ± 0.36,respectively,and the expression in the renal cell carcinoma tissues was significantly higher than that of the adjacent carcinoma tissues( P < 0. 05). The high expression of PVT1 in the renal carcinoma tissues was found in 39 cases of 54 cases,and 15 cases were low expression. The relative expression of PTV1 in renal carcinoma tissues was related with lymph node metastasis and TNM stage( all P < 0. 05),and was not related with sex,age and pathological types( all P > 0. 05).The overall survival time of patients with high expression and low expression of PVT1 in the renal carcinoma tissues was 47.9 ± 9. 4 and 67. 2 ± 3. 8 months,the overall survival time of patients with high PTV1 expression was significantly lower than that of patients with low PTV1 expression( P < 0. 05). Moreover,knockdown PVT1 expression in 786-0 cell line decreased the proliferation activity and enhanced the apoptosis rate( all P < 0. 05). Conclusion PVT1 may promote the occurrence and development of renal carcinoma by affecting the proliferation and apoptosis of renal cells,which can be used as a potential new target for renal cell carcinoma.