榄绿粗叶木茎甲醇提取物对人食管癌EC-9706细胞增殖的影响及其机制探讨

Effect of methanol extract from lasianthus lancilimbus on proliferation of human esophagus carcinoma cells EC-9706

目的探讨榄绿粗叶木茎甲醇提取物(以下简称MESLL)对人食管癌EC-9706细胞增殖的影响及其机制。方法体外培养EC-9706细胞。1观察MESLL对细胞增殖的影响:取对数生长期EC-9706细胞,随机分为MESLL组、环磷酰胺组(CTX)组和空白组。MESLL组分别给予0.01、0.05、0.1、0.2、0.4 mg/m L MESLL干预,CTX组给予1 mg/m L CTX干预,空白组不干预。干预24、48、72 h,检测各组细胞增殖抑制率。2观察MESLL对细胞NO活性及NOS、c GMP含量的影响:取对数生长期细胞,随机分为实验组及对照组。实验组分别置入含0.01、0.05、0.1、0.2、0.4 mg/m L MESLL的培养液培养48 h,对照组常规培养48 h。检测两组细胞上清液NO活性及细胞NOS、c GMP含量。结果 1MESLL对细胞增殖的影响:干预24 h,仅MESLL组0.2、0.4 mg/m L细胞增殖抑制率高于CTX组(P均<0.05);干预48、72 h,MESLL组细胞增殖抑制率随MESLL浓度升高而增加,MESLL组0.1、0.2、0.4mg/m L细胞增殖抑制率均高于CTX组(P均<0.05)。2 MESLL对细胞NO活性及NOS、c GMP含量的影响:随MESLL浓度增加,EC-9706细胞NO活性及NOS、c GMP含量呈降低趋势(P<0.05)。结论 MESLL能抑制食管癌EC-9706细胞增殖,其机制可能与抑制NO/NOS-c GMP通路有关。

Objective To investigate the inhibitory effect of methanol extract from lasianthus lancilimbus ( MESLL) on proliferation of human esophagus carcinoma cells EC-9706 and the mechanism.Methods EC-9706 cells were cultured in vitro.①Observing the effect of MESLL on cell proliferation:the cells in the logarithmic growth phase were divided into MESLL group, cyclophosphamide ( CTX) group and control group.MESLL group was respectively treated with 0.01, 0.05, 0.1, 0.2 and 0.4 mg/mL MESLL, the CTX group was intervened by 1 mg/mL CTX and the control group was not treated.The proliferation inhibiting rates were detected after 24, 48 and 72 h.②Observing the effect of MESLL on NO ac-tivity and the levels of NOS and cGMP:the cells in the logarithmic growth phase were divided into the experimental group and the control group.The experimental group was respectively treated with 0.01, 0.05, 0.1, 0.2 and 0.4 mg/mL MESLL for 48 h, and the control group was not treated.The NO activity and the levels of NOS and cGMP were measured. Results ①After 24 h, the proliferation inhibiting rates treated with 0.2 mg/mL and 0.4 mg/mL MESLL were higher than those of the CTX group (all P<0.05).After 48 and 72 h, tumor inhibiting rate of MESLL group was increased with the increased concentrations of MESLL.The tumor inhibiting rates treated with 0.1 mg/mL, 0.2 mg/mL and 0.4 mg/mL MESLL were higher than those of the CTX group (all P<0.05).②Effect of MESLL on NO activity and the levels of NOS and cGMP:the activity of NO, the levels of NOS and cGMP reduced with the increased concentrations of MESLL ( P<0.05).Conclusions MESLL could inhibit the proliferation of EC-9706 cells, and the mechanism may be related with the inhibition of NO/NOS-cGMP pathway.