肝细胞癌病变肝脏血管铸型标本的制作及意义

Preparation of vascular corrosion casting mould specimen of liver with tumor and its clinical significance

目的制作有肝细胞癌病变的肝脏血管铸型标本,为科研和教学了解肝细胞癌的生长提供形态学依据。方法将肝细胞癌患者手术切除的新鲜成人肝组织用10%醋酸生理盐水插管冲洗,彻底去除肝脏血管内的血液,分别用混合红色、蓝色和黄色油画颜料的过氯乙烯-自凝牙托粉材料填充剂依次灌注肝固有动脉、肝门静脉和肝静脉,通过聚合反应塑化成型,经插管→灌注→腐蚀→冲洗→修整→装瓶密封,制成有肝细胞癌病变的肝脏血管铸型标本进行观察。结果肝细胞癌病变的肝脏血管铸型标本能清晰显示肝门静脉、肝动脉、肝静脉、肝肿瘤及其周围血管分布。显示的血管充盈饱满,粗细、疏密合理,色泽鲜艳,立体感强,精致美观。结论肝细胞癌病变的肝脏可制成血管铸型标本,制成的标本能直观显示肝细胞癌病变的肝脏内血管、肿瘤区域及其周围血管分布。

Objective To prepare the vascular corrosion casting mould specimen of liver with hepatocellular carcinoma and to provide reliable morphological evidence for clinical activity,teaching and scientific research of liver tumor.Methods A fresh hepatocellular carcinoma specimen obtained by surgical resection was injected with 10% acetic acid saline to thoroughly remove blood within the liver. Then the hepatic artery,hepatic portal vein and hepatic vein were perfused with mixtures of perchlorovinyl resin and polymethyl methacrylate resin with red,blue and yellow coloring agent,respectively. Vascular corrosion casting mould specimen of the liver with primary tumor was obtained by intubation,perfusion,corrosion,flushing,trimming and sealing to the container. Results The method could clearly showed the hepatic artery,hepatic portal vein and hepatic venous system and the distribution of blood vessels in and around the tumor area. The vessels were full filling,in reasonable size and density. Meanwhile,they had bright color,a great three-dimensional sense with delicacy. Conclusion The specimen of hepatocellular carcinoma can be made into vascular corrosion casting mould and this method can display its blood vessel,tumor and peripheral vascular distribution.