胃癌组织lncRNA MEG3、GAS5表达变化及其与患者临床病理特征和预后的关系

Expression changes of lncRNA MEG3 and GAS5 in gastric cancer and their relationships with clinicopathological features and prognosis

目的观察胃癌组织长链非编码RNA(lncRNA)母系表达基因3(MEG3)、生长特异抑制物5(GAS5)表达变化,并分析其表达变化与患者临床病理特征和预后的关系。方法选择胃癌组织及其癌旁正常组织各73例份,采用real-time PCR法检测MEG3、GAS5表达。以胃癌组织MEG3、GAS5相对表达量的均值为临界值,高于临界值作为高表达,低于或等于临界值作为低表达。分析胃癌组织MEG3、GAS5表达与患者临床病理特征和预后的关系。结果胃癌组织MEG3、GAS5相对表达量均明显低于癌旁正常组织(P均<0.05)。胃癌组织MEG3、GAS5表达均与肿瘤组织分化程度、临床分期及淋巴结转移有关(P均<0.05)。MEG3高表达者36例、低表达者37例,GAS5高表达者38例、低表达者35例。MEG3、GAS5高表达者术后生存率和生存时间均明显高于MEG3、GAS5低表达者(P均<0.05)。结论胃癌组织MEG3、GAS5低表达,其低表达与胃癌进展和患者预后不良有关。

Objective To observe the expression changes of long non-coding RNA(lncRNA)maternal expression gene 3(MEG3)and growth-specific inhibitor 5(GAS5)in the gastric cancer tissues,and to analyze the relationships between the expression changes and clinicopathological characteristics and prognosis of patients.Methods Seventy-three gastric cancer tissues and 73 adjacent normal tissues were selected.The real-time PCR was used to detect the expression of MEG3 and GAS5.The relationships between the expression of MEG3 and GAS5 in gastric cancer tissues and the clinicopathological characteristics and prognosis of patients were analyzed.Results The relative expression of MEG3 and GAS5 in the gastric cancer tissues was significantly lower than that in the adjacent normal tissues(both P<0.05).The expression of MEG3 and GAS5 in the gastric cancer tissues was correlated with the degree of differentiation,lymph node metastasis,and clinical stage(all P<0.05).According to the mean value of relative expression of MEG3 and GAS5 in gastric cancer tissues,there were 36 cases with high expression of MEG3,37 cases with low expression,38 cases with high expression of GAS5,and 35 cases with low expression.The survival rate and survival time of patients with high expression of MEG3 and GAS5 were better than those with low expression of MEG3 and GAS5(both P<0.05).Conclusion The expression of MEG3 and GAS5 in the gastric cancer tissues is low,which is related to the progression of gastric cancer and prognosis of patients.