胃康3号对功能性消化不良小鼠胃窦、十二指肠及脑组织NT、CGRP受体表达的影响

Effect of Weikang No.3 on expression of NT and CGRP receptors in gastric antrum, duodenum and brain tissues of FD model mice

目的观察胃康3号对功能性消化不良(FD)小鼠胃窦、十二指肠及脑组织神经降压素(NT)、降钙素基因相关肽(CGRP)受体表达的影响,旨在从脑肠轴角度探讨胃康3号治疗FD的作用机制。方法选择昆明种小鼠40只,随机抽取10只作为对照组,剩余小鼠采用不可预知的慢性应激制备FD模型。30只小鼠均制成FD模型,并随机分为模型组、多潘立酮组、胃康3号组。多潘立酮组、胃康3号组分别给予多潘立酮、胃康3号7.56 mL/(kg·d)灌胃,模型组给予等量蒸馏水灌胃,连续灌胃7 d。对照组正常喂养,不建模。末次灌胃次日,麻醉后取胃窦、十二指肠和脑组织,采用免疫组化SP法检测各组织NT、CGRP受体表达。结果模型组胃窦、十二指肠及脑组织NT、CGRP受体相对表达量均明显高于对照组(P均<0.01),胃康3号组、多潘立酮组胃窦、十二指肠及脑组织NT、CGRP受体相对表达量较模型组均明显下降(P均<0.01),但胃康3号组与多潘立酮组不同组织NT、CGRP受体相对表达量比较P均>0.05。结论胃康3号能下调FD小鼠胃窦、十二指肠及脑组织NT、CGRP受体表达,这可能是其治疗FD的作用机制之一。

Objective To observe the influence of Weikang No.3 on the expression of neurotensin(NT)and calcitonin gene-related peptide(CGRP)in the gastric antrum,duodenum and brain tissues of FD model mice,and to explore the mechanism of Weikang No.3 in treatment of FD from the angle of brain gut axis.Methods Forty Kunming mice were selected and 10 were randomly selected as the control group.The remaining mice were subjected to unpredictable chronic stress to prepare the FD models.Thirty FD model were successfully established and they were randomly divided into the model group,domperidone group,and Weikang No.3 group.The mice in the domperidone group and Weikang No.3 group were given domperidone and Weikang No.3[7.56 mL/(kg·d)]by gavage,respectively,and the mice in the model group were given the same amount of distilled water for 7 days.Mice in the control group were fed a normal diet,no modeling.The day after the last gavage,gastric antrum,duodenum and brain tissues were taken after anesthesia,and the expression of NT and CGRP receptors were detected by immunohistochemical SP method.Results The relative expression levels of NT and CGRP receptors in the gastric antrum,duodenum and brain tissues of the model group were all higher than those of the control group(all P<0.01).The relative expression of NT and CGRP receptors in the gastric antrum,duodenum and brain tissues of Weikang No.3 group and domperidone group decreased significantly as compared with that of the model group(all P<0.01).No significant difference was found in the expression levels of NT and CGRP receptors in different tissues between the Weikang No.3 group and domperidone group(all P>0.05).Conclusion Weikang No.3 can downregulate the expression of NT and CGRP receptors in the gastric an-trum,duodenum and brain tissues of FD model mice,which might be one of the mechanisms in treatment of FD.