牡丹皮超临界CO_2萃取物质量标准研究

Study on quality standard of the extracts in Paeonia suffruticosa Andr. by supercritical-CO_2 fluid extraction (SFE)

采用超临界CO2 萃取技术对牡丹皮的有效部位进行萃取 ,并对其萃取物进行了鉴别及含量测定等方面的研究。采用薄层色谱法 (TLC) ,以 ψ(石油醚∶苯∶醋酸乙酯 ) =16∶2 ∶ 2 为展开剂 ,ρ =5 %FeCl3 乙醇溶液为显色剂对牡丹皮超临界CO2 萃取物中的丹皮酚进行鉴别 ;并建立以正十八烷为内标 ,采用SE -3 0 ( 3 0m× 0 .2 5mm× 0 .2 5 μm)石英毛细管色谱柱 ;进样口温度 2 10℃ ,检测器温度 3 0 0℃的气相色谱测定丹皮酚含量的方法。丹皮酚在 ρ =99～ 693 μg/mL浓度范围内 ,峰面积与内标峰面积之比值与浓度呈良好的线性关系 (r =0 .9999) ;平均回收率为 97.12 % ,RSD =1.2 3 % (n =6)。方法准确、简便、灵敏。

The chemical substrates of the curative effect in Paeonia suffruticosa Andr. was extracted by supercritical- CO 2 fluid extraction and the quality standard was studied. Paeonol in extract substrates of Paeonia suffruticosa Andr. was identified by TLC using a mobile phase of petroleum ether,benzene and ethyl acetate ( 16∶2∶2 ) and the color developing reagent of 5% Fecl 3 ethanol. The content of paeonol was determined by capillary gas chromatography with n-octadecane as internal standard. The injector and FID detector temperatures were 210 ℃ and 300 ℃ respectively. The calibration curve was linear in the range of ρ =99～693 μg/mL, r =0 9999. The average recovery was 97.12% (RSD=1.23%,n =6). This determination method was accurate,simple,sensitive and rapid.