妊高征患者血清和脐血中MDA、SOD、维生素E含量的测定

The Change of MDA,SOD and Vitamine E in Umbillcal Blood and Patients with Pregnancy Induced Hypertension

目的 :测定妊高征患者血清和脐血中丙二醛 (MDA)、超氧化物歧化酶 (SOD)及维生素E(VitE)的水平并探讨在妊高征发病中的作用。方法 :分别用硫代巴比妥酸比色法、化学发光分析法和比色法测定 6 4例正常孕妇及5 4例妊高征孕妇血及脐血清中MDA、SOD及VitE水平。结果 :妊高征轻、中重度组较正常同期孕妇MDA含量明显升高 (P <0 .0 1,P <0 .0 1) ,SOD活力及VitE水平明显下降 (P <0 .0 5 ,P <0 .0 1;P <0 .0 1,P <0 .0 1) ,中重度妊高征组与轻度组间各指标有显著差异 (P <0 .0 1) ,脐血各组各参数略低于母血或与其接近 ,两者呈正相关。结论 :提示妊高征患者和新生儿机体氧化 抗氧化平衡失调 ,自由基损伤过程对妊高征发生发展起重要作用。

Objective: To evaluate the role of malonyl dialdohyde (MDA),superoxide dismutase (SOD) and vitamine E(Vit E) in the pathogenesis of pregnancy induced hypertension (PIH). Methods: The maternal serum and cord plasma from 64 cases of normal pregnancy and 54 cases of pregnancy complicated with PIH were measured by TBA method chemoluminescence and colorimetric method. Results: The levels of MDA in the patients with mild, moderate and severe PIH were significantly higher than those of normal pregnante women of the same gestation age (P<0.01,P<0.01). The SOD activity and the level of VitE in the patients with PIH were significatly lower than those of the normal group (P<0.05, P<0.01;P<0.01,P<0.01). There were significantly difference between parameters of the groups with mild PIH compared with the moderate and severe groups. The levels of each parameters in the cord blood were lower than or close to those of maternal blood and showed positive correlation. Conclusion: These means there was imbalance between oxidation and antioxidation in the patients with PIH and their new borns. The process of free radical injury has important effect on the occurance and development of PIH.