嗜盐古菌染色体DNA片段在大肠杆菌中的启动子功能(英文)

Promoter Function of Halobacterium halobium Chromosome DNA Fragment in Escherichia colix

以启动子探针质粒pKK232-8为载体,用微量量热法研究了源自盐生盐杆菌R1染色体的RM13DNA片段在大肠杆菌HB101中的真细菌启动子功能,该启动子RM13 DNA片段的大小为1000bp(碱基对),它能启动探针质粒pKK232-8上的氯霉素乙酰水解酶基因(即:氯霉素抗性基因Cm),氯霉素抗性水平可达到150 mg·L^(-1),抗性水平较高,启动活性较大.研究结果表明,在盐生盐杆菌染色体上可能存在具有双功能或多功能的启动子DNA片段,这对系统发育、微生物遗传学和生物热化学等均有重要的意义.

A promoter-probe plasmid pKK232-8 was used as a vector, which functioned in eubacteria Escherichia (E.) coli HB101. The promoter function of the chromosome DNA fragment from Halobacterium(H.) halobium RI, RM13 DNA fragment, active as eubacteria promoters in E. coli HB101, was studied by microcalorimetry. The promoter is of about 1 000 bp(base pair). It can promote the chloramphenicol acetyltransferase (CAT) gene in plasmid pKK232-8, and the chloramphenicol resistance level is about 150 mg . L-1. The result indicates that there are probably double-function promoters in H. halobium chromosome.