钇离子及其阳离子卟啉配合物与金黄色葡萄球菌的相互作用

Interaction of Y^(3+) and Its Cationic Monoporphyrinate Complex with Staphylococcus aureus

用LKB 2 2 77生物活性检测系统考察了Y3 + ,TMP及其阳离子型钇卟啉配合物 {[Y(TMP) (H2 O) 3 ]Cl ,TMP =5 ,10 ,15 ,2 0 四 (4 甲氧基苯基 )卟啉 }对金黄色葡萄球菌全程代谢作用的影响 ,测定了Y3 + ,TMP和 [Y(TMP) (H2 O) 3 ]Cl对金黄色葡萄球菌作用的产热曲线 ,根据产热曲线求算了金黄色葡萄球菌在Y3 + ,TMP ,[Y(TMP) (H2 O) 3 ]Cl作用下生长代谢的速率常数k1,k2 ,抑制率I1,I2 和半抑制浓度IC150 ,IC250 等热动力学参数 .实验结果表明 :Y3 + ,[Y(TMP) (H2 O) 3 ]Cl对金黄色葡萄球菌的生长代谢有双向调节作用 ,在低浓度下表现为刺激作用 ,高浓度为抑制作用 ,而TMP对金黄色葡萄球菌的生长代谢主要为抑制作用 ,对金黄色葡萄球菌的抑制作用 [Y(TMP) (H2 O) 3 ]Cl>TMP >Y3 + .

DThe biothermochemical characteristics of the actions of Y3+, TMP and their cationic monoporphyrinate complex {[Y(TMP)(H2O)(3)]Cl, TMP = 5,10,15,20-tetrakis(4'-methoxyphenyl)porphyrin} on growth metabolism of Staphylococcus aureus were studied by means of a LKB-2277 Bioactivity Monitor at 37.00 degreesC. The growth rate constants k(1), k(2); inhibiting ratio I(%), the total heat effect (Q(total)) and half inhibition of concentration IC50 (mg(.)L (-1)), respectively were obtained. Experimental results show that Y3+ and [Y(TMP)(H2O)(3)]Cl have the duplex biological effection: they stimulate the growth of the Staphylococcus aureus at lower concentration but inhibit the growth at high concentration. Inhibitory effection of Y3+, TMP and [Y(TMP) (H2O)(3)]Cl is [Y(TMP) (H2O)(3)]Cl > TMP > Y3+.