氯化粪水有机致突变组分的分离

Isolation of Organic Mutagens From Chlorinated Fecal Effluents

目的探讨实验室模拟条件下氯化粪水有机浓集物致突变组分的分离。方法采用Ames试验与硅胶柱分离技术相结合,将采集的粪水经大孔树脂XAD-2/8混合柱吸附,用乙酸乙酯和甲醇进行洗脱,洗脱浓集物用丙酮溶解分别置于大孔硅胶层析柱上过柱,然后用石油醚、二氯甲烷、丙酮、甲醇和水依次对层析柱作径向淋洗展开。用Ames试验对各淋洗液浓集物进行致突变性检测。结果粪水氯化后有机浓集物呈现强致突变性,乙酸乙酯洗脱浓集液(EA)残渣量和比活性高于甲醇洗脱浓缩液(M)。用该程序分离的EA和M的主要活性组分都集中于二氯甲烷和丙酮两个部分,其残渣回收率分别高达96％、100％,重量比活性分别提高了4.06、15.27倍(TA98菌株)和2.85、5.29倍(TA100菌株)。结论该分离程序是有效的。

Objective To isolate mutagenic components in chlorinated night stool in lab simulative condition. Methods Both Ames test and silica gel chromatographic analysis were used .The night stool samples were concentrated by using XAD-2/8 resin filled columns, and washed by ethyl acetate and methanol. The condensate in elution were dissolved in acetone and isolated through silica gel columns eluted by petroleum ether, dichloromethane, acetone ,methanol and water respectively, the muta-genicity of organic condensates in elution were tested by Ames test. Results The residue amount and mutagenicity in ethyl acetate elution were much higher than those in methanol elution, and the main mutagenic components existed in both dichloromethane and acetone separating liquids. The recovery rate of residual was 96% of the condensate in dichloromethane and acetone separating liquids of ethyl acetate elution and 100% in methanol elution. The specific activities of weight of dichloromethane and acetone separating liquid of ethylacetate elution and methanol elution increased by 4.06, 15.27 times for TA98 strain, and by 2.85, 5.29 times for TA100 respectively Conclusion The results showed that the separating procedures were effective.