昆虫杆状病毒表达载体系统在疫苗研究中的应用进展

Progress in vaccine development based on baculovirus expression vector system

昆虫杆状病毒表达载体系统(Baculovirus expression vector system,BEVS)已成功应用于多种蛋白的表达,并为疫苗开发提供了充足的原材料。相比其他表达系统,BEVS具有许多优势:杆状病毒专一寄生于无脊椎动物,安全性高;重组蛋白表达水平高;可对重组蛋白进行正确折叠和翻译后修饰,获得具有生物活性的蛋白;适应于多基因表达如病毒样颗粒(Virus-like particle)的复杂设计;适用于大规模无血清培养等。为了更好地理解BEVS在疫苗研究中的应用前景,文中将从BEVS的发展及其在疫苗研究中的应用等方面进行综述。

Baculovirus expression vector system(BEVS)has been successfully applied to the over-expression of various proteins,thus providing sufficient materials for vaccine research.Compared to other systems,BEVS has many advantages:baculovirus solely being parasitic in invertebrates,the resultant products conferring high safety to mammalian,high expression level of recombinant proteins,preferable folding for eukaryotic protein,proper post-translational modification required for biological function,suitable for multiple genes co-expression and large-scale production with serum-free culture media.To better understand the advantages and prospective of BEVS for the vaccine research,this article will review the development of BEVS and its application on vaccine research.