原位显微镜在细胞生物量在线监测中的发展与应用

Development and application of in-situ microscopy in on-line monitoring of cell biomass

随着现代生物技术的快速发展,生物发酵过程在工业生产中的重要性日益增加。为获得质量稳定的发酵产品,通常需要对发酵过程进行监测与调控。生物量可以直接反映生物反应器中细胞代谢的主体——细胞的生长状况,因此实现生物量的在线监测对发酵过程的调控具有重要意义。原位显微镜是一项非侵入式的、基于图像分析的技术,可以实时监测生物过程中的细胞量。文中就原位显微镜的发展及其在细胞生物量实时监测中的应用进行了综述。

With the rapid development of modern biotechnology,fermentation process is increasingly important in industrial production.To guarantee the stability of products,fermentation process should be elaborately monitored and controlled.Biomass is an important parameter for on-line monitoring in bioprocesses because biomass can reflect cell growth in a bioreactor directly.In-situ microscope,a non-invasive and image-analysis based technology,can real-time monitor cells in biological process.This review summarizes the development and application of in-situ microscopy in biomass monitoring.