盐酸小檗碱对急性脑梗死患者血清中HIF-1α和Caspase-3水平影响的多中心研究

A multicenter study effect of berberine hydrochloride on serum HIF-1α and Caspase-3 levels in patients with acute cerebral infarction

目的通过多中心研究,探究盐酸小檗碱对急性脑梗死患者血清中HIF-1α、Caspase-3水平的影响。方法选取2012年1月~2014年1月河北工程大学附属医院、邯郸市第一医院、邯郸市中心医院神经内科收治的急性脑梗死患者共126例,采用随机数字表分为实验组和对照组。对照组63例,在常规治疗基础上静脉点滴奥扎格雷钠注射液80 mg,2次/天;静脉点滴小牛血去蛋白注射液800 mg,1次/天;同时,每晚口服阿司匹林片100 mg,1次/天,7 d为1疗程。实验组63例,在常规治疗的基础上加用盐酸小檗碱片0.7 g,口服,3次/天,7 d为1疗程。1个疗程结束后,分别观察急性脑梗死患者治疗前后血清中HIF-1α水平、血清中Caspase-3水平。结果治疗后2组血清中HIF-1α水平均有所降低,但实验组血清中HIF-1α水平(733.49±225.01)ng/mL较对照组(975.32±311.35)ng/mL降低更加明显,差异有统计学意义(P<0.05)。治疗后2组血清中Caspase-3水平均有所降低,但实验组血清中Caspase-3水平(11.88±2.66)ng/mL较对照组(12.51±3.77)ng/mL降低更加显著,差异有统计学意义(P<0.05)。结论盐酸小檗碱可有效改善急性脑梗死患者血清中HIF-1α、Caspase-3水平,降低急性脑梗死患者病死率,可积极应用于急性脑梗死患者的恢复治疗。

Objective To explore the effect of berberine hydrochloride on serum HIF-1α,Caspase -3 levels in patients with acute cerebral infarction with a multicenter study.Methods 126 patients with acute cerebral infarction from January 2012 to January 2014 were selected in the Affiliated Hospital of Hebei Engineering University,the First Hospital of Handan City,Handan Central Hospital were divided into experimental groups and control group using random number table.Control group had 63 cases,were given 80 mg ozagrel sodium injection intravenously,twice a day,and 800 mg calf blood protein injection intravenously,once per day on the basis of conventional treatment;at the same time,100 mg of oral aspirin tablets were taken,once per night,7 days for a course of treatment.Experimental group were given berberine hydrochloride on the basis of conventional treatment,0.7 g orally,three times a day,7 days for a course of treatment.After a course of treatment,serum HIF-1α,Caspase-3 levels in patients with acute cerebral infarction were observed before and after treatment.Results Serum HIF-1αlevels in two groups were reduced after treatment,and its level in experimental group[(733.49 ±225.01)ng/mL]was lower than that in control group [(975.32 ±311.35)ng/mL],the difference was statistically significant (P<0.05 ).Serum Caspase-3 levels in two groups after treatment were decreased,and its level in experimental group [(1 1.88 ±2.66 )ng/mL]was lower than the control group [(12.51 ±3.77)ng/mL],the difference was statistically significant (P <0.05).Conclusion Berberine hydrochloride could improve the serum HIF-1α,Caspase-3 levels in patients with acute cerebral infarction,reduce the fatality rate,and could be actively used in the recovery of patients with acute cerebral infarction treatment.