miR-199a对膀胱癌抑制作用的研究

Inhibitory effect of miR-199a on bladder cancer

目的研究miR-199a对膀胱癌的抑制作用。方法按处理方法不同将T24膀胱癌细胞分为空白对照组(control group)、pre-scamble转染组(pre-scamble group)、pre-miR-199a转染组(pre-miR-199a group)。采用MTT法检测转染miR-199a对细胞增殖的影响,用流式细胞仪检测转染miR-199a后对细胞凋亡及细胞周期的影响,Transwell侵袭实验检测细胞侵袭能力。结果 premiR-199a group OD值(0.436±0.042)显著低于control group(0.634±0.020)和pre-scamble group(0.601±0.059)(P<0.05)。premiR-199a group细胞凋亡率为(19.25±1.57)%,显著高于control group(10.19±0.98)%和pre-scamble group(12.27±1.38)%(P<0.05)。Control group、pre-scamble group和pre-miR-199a group的G1期细胞百分比分别为45.09%、47.57%、58.62%,转染pre-miR-199a使细胞停滞在G1期。pre-miR-199a group细胞透过滤膜的平均个数为(46.00±1.58)个,显著低于control group(67.00±1.58)个、pre-scamble group(61.20±1.30)个(P<0.05)。结论 miR-199a能抑制膀胱癌细胞的生长。

Objective To study the inhibitory effect of miR-199a on bladder cancer.Methods T24 bladder cancer cells were divided into control group, pre-scamble group and pre-miR-199a group according to different treatment.Cell proliferation was assayed by MTT, cell apoptosis and cell cycle by flow cytometry, and cell invasion by Transwell.ResuIts The OD value of pre-miR-199a group (0.436 ±0.042) was significantly lower than that of control group (0.634 ±0.020) and pre-scamble group (0.601 ±0.059)(P<0.05).Cell apoptosis of pre-miR-199a group(19.25 ±1.57)% was higher than that of control group(10.19 ±0.98)% and pre-scamble group(12.27 ±1.38)%(P<0.05).The cell ratio in G1 phase of control group、pre-scamble group and pre-miR-199a group was 45.09%, 47.57%, and 58.62%, respectively.The cell cycle arrested in G1 phase after transfected with pre-miR-199a.The cells migration number of pre-miR-199a group (46.00 ±1.58) was lower than those of control group(67.00 ±1.58) and pre-scamble group(61.20 ±1.30)(P<0.05).ConcIusion MiR-199a could inhibit the growth of bladder cancer cells.