褥疮冰蜜湿敷剂中单味药的抗菌实验研究

Study on antimicrobial effects of single herbs in wet Ruchuang Bingmi hydropathic compress agent

目的研究褥疮冰蜜湿敷剂中冰片、蜂蜜、庆大霉素单味药间的抗菌作用。方法用金黄色葡萄球菌(Staphylococcus aureus,S.aureus)、大肠杆菌(Escherichia coli,E.coli)、铜绿假单孢杆菌(Pseudomonas aeruginosa,P.aeruginosa)分别制作三期感染褥疮动物模型,以凡士林为对照组,分为12组,即凡士林-S.aureus组,凡士林-E.coli组,凡士林-P.aeruginosa组;蜂蜜-S.aureus组,蜂蜜-E.coli组,蜂蜜-P.aeruginosa组;冰片-S.aureus组,冰片-E.coli组,冰片-P.aeruginosa组;庆大霉素-S.aureus组,庆大霉素-E.coli组,庆大霉素-P.aeruginosa组;每组6只大白兔,分别用蜂蜜、冰片、庆大霉素制作成换药纱布治疗褥疮,取治疗前后组织做菌落计数观察。结果冰片组(F=11.059,P<0.01),表明冰片培养的各组计数有差别;时间×菌种(F=11.281,P=0.009),2组之间相互作用不明显;庆大霉素组(F=7.99,P=0.000),表明庆大霉素培养的各组计数有差别;时间×菌种(F=12.531,P<0.07),表明2组之间相互作用明显。冰片对P.aeruginosa无抗菌作用,对S.aureus和E.coli有一定的抗菌作用;庆大霉素对上述3种细菌的抗菌作用良好,对P.aeruginosa的抗菌作用尤为显著。结论湿敷剂中单味药庆大霉素的抑菌作用优于冰片,蜂蜜无抗菌作用。

Objective To study the antibacterial effect of borneol, honey, gentamicin in Ruchuang Bingmi hydropathic compress agent. Method Staphylococcus aureus( S. aureus),Escherichia coli( E. coli),Pseudomonas aeruginosa( P. aeruginosa) were used to produce the third phase of infection decubitus animal models,respectively. Vaseline as the control group,divided into 12 groups: Vaseline-S. aureus,Vaseline-E. coli,Vaseline-P. aeruginosa; Honey-S. aureus,Honey-E. coli,Honey-P. aeruginosa; Borneol-S. aureus; Borneol-E. coli; Borneol-P. aeruginosa; GentamicinS. aureus; Gentamicin-E. coli; Gentamicin-P. aeruginosa; 6 rabbits in each group. Honey,borneol,gentamicin was made into a gauze in treatment for decubitus. Organizations do strain identification and colony counts was observed before and after taking the treatment. Results Borneol group( F =11. 059,P < 0. 01).,there was differences of each groups count cultured by borneol; Time × Strains( F = 11. 281,P = 0. 009),there was no significant interaction between the two groups; gentamicin( F = 7. 99,P = 0. 000),gentamicin culture showed a difference in each group count; Time × Strains( F =12. 531,P < 0. 07),interaction between the two groups showed significant. Borneol has no antibacterial effects on P. aeruginosa,had a certain antibacterial activityon S. aureus and E coli; gentamicin had good antibacterial effect on the three kinds of bacteria,and against P. aeruginosa was particularly significant. Conclusion The antibacterial activity of gentamicin is better than single herbs borneol in Ruchuang Bingmi hydropathic compress agent,honey has no antibacterial effect.