黄芩苷对鱼藤酮致BV2细胞激活损伤的影响

Effect of baicalin on BV2 cell activation damage caused by rotenone

目的观察黄芩苷对鱼藤酮致BV2细胞激活损伤的影响。方法采用MTT法测定鱼藤酮染毒BV2细胞存活率、ICC法测定细胞OX42表达变化、荧光分光光度法测定细胞内Ca2+含量、ICP-AES测定细胞内铁含量以及采用相关试剂盒测定胞外H2O2和胞内MDA、NO、NOS的含量。结果鱼藤酮染毒BV2细胞存活率降低,细胞标记蛋白OX42、细胞内Ca2+含量、细胞内铁含量显著增加(P<0.05),造成细胞产生氧化损伤。黄芩苷可减少鱼藤酮染毒BV2细胞OX42表达,增加细胞存活率、减少细胞内铁含量、减少细胞H2O2释放,减少氧化损伤(P<0.05)。结论黄芩苷抑制鱼藤酮染毒所致BV2的激活和损伤,具有神经保护作用;其保护作用机制与减少铁进入细胞有关。

Objective To observe Effect of baicalin on BV2 cell activation damage caused by rotenone.Methods Rotenone infected BV2 cell viability were detected by MTT assay, and OX42 expression by ICC method assay, the inner cell Ca2 + content by fluorescence spectrophotometry, intracellular iron content by ICP-AES when low concentrations iron of load without damage in cells and extracellular H2 O2 and intracellular content of MDA, NO, NOS with related kits.ResuIts Rotenone exposure BV2 cell reduced cell viability, the expression of cell marker protein OX42 and intracellular Ca2 +content and intracellular iron content were all significantly increased(P<0.05), resulting in oxidative damage to cells.Baicalin reduced OX42 expression of rotenone exposure BV2 cell, increased cells survival, reduced intracellular iron content and H2 O2 release and oxidative damage of BV2(P<0.05).ConcIusion Baicalin inhibits BV2 activation and damage by rotenone-induced, and it has neuroprotective effects.The protection mechanism may relate to reduce iron into cells .