慢性淋巴细胞性白血病免疫分型的探讨

AN APPROACH TO THE IMMUNOLOGIC TYPES OF CHRONIC LYMPHOCYTIC LEUKEMIA

本文采用六项指标对12例临床已确诊为慢性淋巴细胞性白血病(简称慢淋)活动期患者进行免疫分型。分型结果7例属 B 细胞型,3例 T 细胞型,2例 Null 细胞型。还比较了各项分型技术,认为 E_s 是检测 T 细胞的特征性标记,SmIg 是 B 细胞的特征性标记。此外 ANAE 也是检测 T 细胞比较可靠、简单易行的方法。

Peripheral blood lymphocytes from 12 adult patients with chronic lymphocytic leu-kemia(CLL)were studied to determine their immunologic types.T-cell markers wereidentified by sheep red cell rosette formation(Es)test and B-cell markers by mousered cell rosette formation(Em)test as well as surface membrane immunoglobulin(Sm-IgG and SmIgM)demonstration.Acid α-Naphthyl Acetate Esterase(ANAE)activityand electrophoretic mobility of lymphocytes were also estimated.Seven patients had Bmarkers(B-CLL),three had T markers(T-CLL),and two had neither B nor T mark-er(Null cell CLL).There were different clinical manifestations,hematological find-ings,etc.between T-CLL and B-CLL.In addition to Es and SmIg,ANAE activitystaining is also a simple and reliable method in differentiating the immunologic typesof lymphocytes.Because not all B-cells possess sufficient surface immunoglobulin to bedetermined by immuno-fluorescent technique,the authors suggest that the Em test shouldbe used as a supplement in determining the surface marker of B-cells,The usefulnessof electrophoretic mobility test in differentiating the lymphocytes requires further in-vestigation.