薯蓣皂苷元对PC12细胞氧化损伤的保护作用

Protective Effect of Diosgenin on Oxidative PC12 Cells Damage

目的:探讨薯蓣皂苷元(diosgenin,DG)对H2O2诱导损伤的PC12细胞的保护作用。方法:用H2O2损伤PC12细胞建立氧化应激损伤细胞模型,在电子显微镜下观察其细胞形态学特征,并通过MTT法检测不同浓度DG对正常PC12细胞和模型细胞增殖的影响;应用流式细胞术检测DG对模型细胞凋亡率和细胞周期的影响。结果:与正常组比较,不同浓度H2O2(100、200、400μmol/L)损伤组PC12细胞生存率均显著下降(P<0.01),DG 3个浓度组(1.25、2.5、5.0μmol/L)PC12细胞生存率均显著提高(P<0.01)。与模型组比较,DG 3个浓度组(1.25、2.5、5.0μmol/L)PC12细胞生存率均显著提高(P<0.01);2.5μmol/L的DG可显著抑制H2O2诱导的PC12细胞凋亡(P<0.01),在细胞周期上表现为G0~G1期细胞减少,同时S期增多。结论:DG能促进PC12细胞增殖,对H2O2氧化损伤的PC12细胞具有保护作用。

Objective: To investigate the protective effects of diosgenin( DG) on oxidative PC12 cells damage induced by H2O2. Methods: PC12 cells induced by H2O2 were produced for oxidative stress damage cell models. Electron microscope was applied to observed morphological feature of the cells. MTT assay was performed to assess the survival rates of different concentrations of DG on normal PC12 cells and model cells. Apoptosis and cell cycle were examined by flow cytometric analysis. Results: Compared with the normal group,the cell viability in H2O2( 100,200,400 μmol/L) groups were reduced significantly( P < 0. 01),and the cell viability in the DG( 1. 25,2. 5,5. 0 μmol / L) were all significantly increased( P <0. 01). Compared with the model group,the cell viability in the DG( 1. 25,2. 5,5. 0 μmol / L) groups were significantly increased( P < 0. 01),and apoptosis of the DG( 2. 5 μmol / L) group was inhibited significantly compared with the model group( P < 0. 01). The number of cells at G0 ~ G1 phase was decreased and at S-phase was increased on cell cycle.Conclusion: DG could promote the multiplication of PC12 cells,and display protective effects on the PC12 cells injury induced by H2O2.