温和灸肾俞穴对亚急性衰老大鼠免疫功能的影响

Effects of Mild-warm Moxibustion on Shenshu Acupoint on Immune Function in Subacute Aging Rats

目的:观察温和灸肾俞穴对亚急性衰老大鼠免疫功能的影响。方法:将雄性SD大鼠随机分成正常组、模型组、艾灸组3组,每组8只。模型组、艾灸组通过25%D-半乳糖125 mg·kg-1·d-1颈背部皮下注射制备亚急性衰老大鼠模型,正常组注射等体积的0.9%Na Cl溶液,均每天1次,连续注射40 d。艾灸组大鼠在造模同时,艾灸双侧肾俞穴,每天1次,每次5 min,连续艾灸40 d;模型组和正常组每天肾俞穴做抓取动作5 min。40 d后采用ELISA方法检测各组大鼠血清IgA、IgM、IgG的含量并测算胸腺、脾脏指数。结果:与正常组比较,模型组大鼠血清IgA、IgM、IgG的含量均明显降低,差异均具有统计学意义(P<0.05);与模型组比较,艾灸组血清IgA、IgM、IgG的含量均明显升高,差异具有统计学意义(P<0.05)。模型组胸腺、脾脏指数均较正常组明显降低(P<0.05);艾灸组胸腺、脾脏指数均较模型组明显升高(P<0.05)。结论:温和灸肾俞穴能全面调节机体免疫系统,可能有助于延缓机体衰老进程。

Objective: To observe the effects of mild-warm moxibustion on Shenshu acupoint(BL23) on immune function in subacute aging rats. Methods: Male SD rats were randomly divided into the normal group,model group and moxibustion group,8 rats in each group. The subacute aging rat model was established by subcutaneous injection of 25% D-galactose at daily dose of 125 mg/kg at neck back of rats in the model group and moxibustion group,and the normal group was treated with0. 9% Na Cl solution at equal volume by injection. The modeling course in both groups was once a day for continuious 40 days.At the same time,the moxibustion group was treated with moxibustion on bilateral Shenshu acpoiont for 5 minutes per time,the moxibustion course was once a day for continuious 40 days,and the model group and normal group were treated with grasping action on Shenshu acpoiont for 5 minutes everyday. After 40 days,the contents of serum IgA,IgM and IgG in each group were detected by ELISA,and the indexes of thymus and spleen were calculated. Results: Compared with the normal group,the contents of serum IgA,IgM and IgG in the model group were all obviously decreased with statistically significant differences(P < 0. 05); Compared with the model group,the contents of serum IgA,IgM and IgG in the moxibustion group were all obviously increased with statistically significant differences(P < 0. 05). Compared with the normal group,the indexes of thymus and spleen in the model group were both obviously decreased(P < 0. 05); Compared with the model group,the indexes of thymus and spleen in the moxibustion group were both obviously increased(P < 0. 05). Conclusion: Mild-warm moxibustion on Shenshu acupoint can fully regulate the body immune system,which may help to slow down the aging process.