维吾尔族类风湿关节炎患者血清、关节滑液中RANTES水平的变化及其临床意义

Detection of RANTES in Serum and Synovial Fluid in Uighur Patients with Rheumatoid Arthritis: its Correlation with Disease Activity

为探讨血清、关节滑液中调节活化正常T细胞表达和分泌因子(RANTES)与维吾尔族类风湿关节炎(RA)患者与骨关节炎(OA)疾病活动的相关性。选取已经确诊的维吾尔族RA患者60例、OA患者30例和对照组30例作为研究对象。采用酶联免疫法(ELISA)检测血清及关节滑液中RANTES水平,同期记录相关临床资料及实验室指标。用SPSS19.0进行统计学分析。结果显示RA患者血清、关节滑液中RANTES水平均明显高于OA组和对照组;RA患者关节滑液中RANTES水平较血清中明显增高(t=-2.58,P=0.01)。RA患者血清中RANTES水平与CRP、抗CCP抗体、关节肿胀指数呈正相关(Spearman相关系数r值分别为0.41,0.30,0.29);RA患者关节滑液中RANTES水平与CRP、关节肿胀指数呈正相关(Spearman相关系数r值分别为0.03,0.01)。由此可知,维族RA患者血清、关节滑液中的RANTES水平增高,病变关节组织是RANTES产生的主要部位;血清RANTES可以作为判断维吾尔族类风湿关节炎患者疾病活动性的指标。

To explore the normal T cell expressed and secreted( RANTES) levels in serum and synovial fluid( SF) from Uighur patients with rheumatoid arthritis( RA) and its correlation with disease activity.The serum and SF samples colected from 60 patients with RA,30 patients with OA and 30 control group were collected and studied separately and RANTES in serum and SF were quantified by ELISA.Serum RANTES level was higher in RA than that in OA and control group.The concentration of RANTES was higher in SF than in serum in RA.Serum RANTES level was correlated significantly with CRP,CCP and joint swelling index.Serum RANTES level was correlated significantly with joint swelling index,ESR and CRP.Uighur RA patients with higher level of RANTES in the serum,synovial joints,which mainly secreted by the lesion joint tissues.Serum RANTES can serve as a judge index of Uygur patients with rheumatoid arthritis disease.