甘草渣多糖的大孔树脂分离纯化及抗氧化活性的研究

Purification of Polysaccharide from Licorice Residue by Macroporous Resins and Investigation of its Antioxidant Activity

为探讨甘草渣中多糖的分离纯化条件及抗氧化活性,进行了大孔树脂的选择实验研究,并由大孔树脂动态吸附实验及动态洗脱实验研究确定了HPD-722大孔树脂分离纯化甘草渣多糖的最佳条件,并以维生素C作为对照,对甘草渣多糖清除DPPH自由基和羟基自由基的能力进行了检测。检测与分析结果表明:HPD-722树脂对甘草多糖的吸附率为73.25%,解吸率为86.59%,适合于甘草多糖的纯化;甘草渣多糖最佳分离条件为:上样液甘草多糖浓度4.12 mg/m L、上样量2 BV、上样流速2 BV/h,洗脱剂为50%乙醇,洗脱流速3 BV/h,洗脱剂用量3BV,在最佳条件下甘草多糖的纯度由纯化前的7.64%提高为51.65%;通过抗氧化性实验显示甘草多糖具有较强的抗氧化性,能清除DPPH自由基和羟基自由基,是一种很好的天然抗氧化剂。

For researching the purification process and the antioxidant activity of the polysaccharide from Licorice residues, the macroporous resins was chosen to study its selectivity, and the best condition was determined by dynamic adsorption and desorption experiments of HPD- 722 resin for the purification of the polysaccharide. At the same time, compared with vitamin C,the antioxidant activity of polysaccharide was examined by DPPH radical and hydroxyl radical scavenging activity. The results showed that HPD- 722 was appropriated to purify polysaccharide from licorice with the adsorption rate of 73.25% and the desorption rate of 86.59%. The optimal conditions for purification as followed: the loading concentration of Polysaccharide was4.12 mg/m L, the loading volume was 2 BV, the loading rate was 2 BV/h, the elution was 50% ethanol, the elution rate was 3BV/h and the elution volume was 3 BV. Under the optimized conditions, the purity of polysaccharide was increased from7.64% to 51.65%. The experimental data of antioxidant also showed that the polysaccharide had a high antioxidant activity that could scavenge DPPH radical and hydroxyl radical which would become a good natural antioxidant.