温肾健脾方对腹泻型肠易激综合征大鼠CCK、MOT表达的影响

Warming Kidney and Tonifying Spleen Interventions' effects on the colonic mucosa and ileocecal CCK and MOT of IBS-D Rats

目的观察温肾健脾方对腹泻型肠易激综合征(IBS-D)大鼠结肠黏膜及回盲部黏膜层胆囊收缩素(CCK)、胃动素(MOT)表达的影响,从实验角度探索该方对IBS-D的疗效及作用机制。方法 48只大鼠随机分为正常组、模型组、温肾健脾方高剂量组、温肾健脾方中剂量组、温肾健脾方低剂量组和对照组等6组,各组8只。参照AL-Chaer造模方法建立IBS-D大鼠模型。造模结束后,治疗组分别予温肾健脾方免煎剂高、中、低剂量灌胃治疗,对照组予四神丸方免煎剂灌胃,模型组予等体积0.9%Na Cl溶液灌胃,给药2周。观察各组大鼠一般情况,并采用免疫组化法检测结肠黏膜及回盲部黏膜层CCK、MOT表达情况。结果治疗后各组大鼠一般情况均有不同程度的好转,温肾健脾方高、中剂量组大鼠的改善情况优于温肾健脾方低剂量组和对照组。与正常组比较,模型组大鼠结肠黏膜、回盲部黏膜层CCK、MOT表达升高,差异有统计学意义(P<0.05)。治疗后,与模型组比较,温肾健脾方高、中剂量组及对照组大鼠结肠黏膜、回盲部CCK、MOT表达降低,温肾健脾方高、中剂量组结肠黏膜、回盲部CCK、MOT表达低于温肾健脾方低剂量组,差异均有统计学意义(P<0.05);温肾健脾方高、中剂量组结肠黏膜、回盲部CCK表达低于对照组,差异有统计学意义(P<0.05)。结论温肾健脾方对IBS-D大鼠有较好的治疗作用,其治疗机制可能是通过调控结肠黏膜、回盲部黏膜层CCK、MOT的表达而起到调节胃肠道激素的作用。

Objective To observe the effects of Warming Kidney and Tonifying Spleen interventions on IBS-D rats' colonic mucosa,ileocecal CCK and MOT to probe into the underlying mechanism from the gastrointestinal hormone perspective.Methods 48 rats were randomly divided into normal group,model group,Warming Kidney and Tonifying Spleen high,middle and low dose groups,and control group,8 in each group.Referencing AL-Chaer modeling,made a duplicate IBS-D rat model.After that,the treatment groups were treated with high dose,middle dose and low dose Decocting-free Warming Kidney and Tonifying Spleen formula decoction,while the control group was treated with Decocting-free 'Sishen Pill',and the model group was treated with equal quantity of normal saline irrigation,2 weeks for each group.The general situation of rats in each group was observed,and immunohistochemistry analysis was employed to observe the changes in their colonic mucosa,ileocecal CCK and MOT.Results After the treatment,the general situation of rats in each group became better to various degree,and the situation of rats in the Warming Kidney and Tonifying Spleen high and middle groups was better than that of the low dose and control groups.Compared with normal group,the expression of colonic mucosa,ileocecal CCK and MOT in the rats in model group increased,and there was significant statistic difference(P < 0.05).Compared with model group after the treatment,the expression of colonic mucosa,ileocecal CCK and MOT in the rats in the Warming Kidney and Tonifying Spleen high,middle and control groups decreased.Compared with low dose group,the expression of colonic mucosa,ileocecal CCK and MOT in the rats in the Warming Kidney and Tonifying Spleen high,middle groups decreased,and there was significant statistic difference(P < 0.05).Conclusion Warming Kidney and Tonifying Spleen interventions proved to be effective to reduce the quantity of colonic mucosa and the amount of released ileocecal CCK and MOT,which in turn reduces the intestinal sensitivity and also accelerate the degradation of CCK and MOT present in their body.