复方透骨草溶液抑菌和抗炎作用的实验研究

Experimental study on bacteriostatic and anti-inflammatory effects of Compound Tougucao Solution

目的评价复方透骨草溶液的抑菌和抗炎作用,初步探索其抗炎的作用机制,为临床应用提供实验依据。方法采用体外抑菌实验,观察复方透骨草溶液对常见皮肤癣菌和酵母菌的抑制作用;采用二甲苯致小鼠耳肿胀、角叉菜胶致大鼠足趾肿胀的模型,观察复方透骨草溶液和地塞米松磷酸钠注射液外用涂抹的抗炎作用,并检测大鼠足趾致炎4 h后组织中的白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)的水平。结果体外抑菌实验表明,复方透骨草溶液对皮肤癣菌和酵母菌的最小抑菌浓度(MIC)分别为原液浓度的12.5%和25.0%;其能降低二甲苯致小鼠耳廓的肿胀度,高剂量给药组与阴性对照组比较差异有统计学意义(P<0.01);对角叉菜胶致大鼠足趾肿胀有明显的减轻作用,给药组与阴性对照组比较差异均有统计学意义(P<0.01);能显著降低大鼠足趾肿胀损伤后组织中致炎因子IL-1β的表达,给药组与阴性对照组比较差异均有统计学意义(P<0.05,P<0.01)。结论复方透骨草溶液具有显著的抑菌和抗炎作用,并通过降低组织中的炎症因子IL-1β的水平,从而抑制炎症发展的过程。

Objective To evaluate the bacteriostatic and anti-inflammatory effects of Compound Tougucao Solution and explore the mechanism of anti-inflammation,provide the experimental basis for its clinical application. Methods The bacteriostatic effects of Compound Tougucao Solution on dermatophytes and saccharomycetes were observed by bacteriostatic experiment in vitro. The mice model of dimethylbenzene-induced ear edema and rat model of carrageenan-induced paw swelling were established to observe the anti-inflammatory effects of Compound Tougucao Solution and Dexamethasone Sodium Phosphate Injection with external application. 4 hours after the inflammation induced in rat paw,the levels of interleukin(IL)-1β and tumor necrosis factor(TNF)-α in the tissue were determined.Results The bacteriostatic experiment in vitro showed that the minimum inhibitory concentration(MIC) of Compound Tougucao Solution against dermatophytes and saccharomycetes were 12. 5% and 25. 0% of original solution,respectively. Compound Tougucao Solution could reduce the swelling degree of dimethylbenzeneinduced ear edema in mice,and there was significant difference between the Compound Tougucao Solution group with high-dose and negative control group(P <0. 01). Compound Tougucao Solution could obviously alleviate the swelling degree of carrageenan-induced paw swelling in rats,and there were significant differences between the Compound Tougucao Solution groups with different doses and negative control group(P < 0. 01). Compound Tougucao Solution could decrease the level of IL-1β in the tissue of rat swelling paw,and there were significant differences on IL-1β level between the Compound Tougucao Solution groups with different doses and negative control group(P < 0. 05,P < 0. 01). Conclusion Compound Tougucao Solution shows significant bacteriostatic and antiinflammatory effects,and it plays a role in inhibiting the development of inflammation by decreasing the level of IL-1β in tissue.