夏枯草五倍子抑菌洗液的质量控制方法及抑菌作用研究

Study on quality control methods and bacteriostatic effects of Xiakucao( Prunellae Spica)-Wubeizi( Acanthopanacis Cortex) bacteriostasis lotion

目的建立夏枯草五倍子抑菌洗液的质量控制方法,并考察其对妇科常见菌的体外抑菌作用。方法采用高效液相色谱法测定夏枯草五倍子洗液中主要活性成分没食子酸的含量,色谱柱为Alltima C_(18)(4.6 mm×250 mm,5μm),流动相为0.1%磷酸甲醇溶液-0.1%磷酸溶液(21∶79)等度洗脱,柱温30℃,流速1.0 ml/min,检测波长273 nm,进样量10μl;采用药敏纸片法及抑菌性能测试对洗液抑菌作用进行效果验证。结果没食子酸在0.015 g/L^0.245 g/L范围内线性良好,r值为1,加样回收率为95.46%。体外抑菌试验显示,夏枯草五倍子洗液对金黄色葡萄球菌及大肠埃希氏菌具有良好的抑菌作用。结论高效液相色谱法测定洗液中有效成分没食子酸作为质量控制方法可靠;夏枯草五倍子洗液对妇科常见菌的体外抑菌作用试验表明其为一种有效的阴部抑菌制剂。

Objective To establish the quality control methods for Xiakucao( Prunellae Spica)-Wubeizi( Acanthopanacis Cortex) bacteriostasis lotion,and study its bacteriostatic effects on common gynecological bacteria in vitro. Methods The content of gallic acid,the main active component of the lotion,was detected by high performance liquid chromatography( HPLC). The separation was performed on an Alltima C_(18) column( 4.6 mm !250 mm,5 μm) by gradient elution with the mobile phases of 0.1% phosphoric acid dissolved methanol-0.1% phosphoric acid( 21: 79) at a flow rate of 1.0 ml/min. The temperature of the column was 30 ℃ and the injection volume was 10 μl. The detection wavelength was 273 nm. The bacteriostatic effect of the lotion was verified by Kirby-Bauer susceptibility disk tests and bacteriostasis test. Results The linear curve of gallic acid fitted well in the range of 0.015 g/L-0. 245 g/L( r = 1). The average recovery was 95. 46%.Bacteriostasis tests in vitro showed good bacteriostatic activity of Xiakucao-Wubeizi lotion against Staphylococcus aureus and Escherichia coli. Conclusion Determination of gallic acid in the lotion by HPLC is reliable for quality control. Bacteriostasis tests on common gynecological bacteria in vitro indicate that Xiakucao-Wubeizi lotion is an effective pudenda bacteriostasis agent.