少腹逐瘀汤不同部位的提取及其对子宫内膜异位症患者在位内膜细胞的影响

Extraction of different parts of Shaofu Zhuyu Decoction and effects on eutopic endometrial cells in patients with endometriosis

目的确定少腹逐瘀汤(SF)各分离部位(SF-1~SF-7)中潜在的化学成分及含量并观察其对子宫内膜异位症患者在位内膜细胞的影响。方法利用水蒸气蒸馏法、煎煮法、醇沉法及D101大孔吸附树脂分离纯化制备各部位样品(SF-1~SF-7),并采用高效液相色谱法进行定性和定量分析,气相质谱联用分析挥发油成分。通过培养子宫内膜异位症患者的在位内膜细胞对少腹逐瘀汤各提取部位进行活性筛选。结果对少腹逐瘀汤不同提取部位进行分离,确定了不同提取部位的主要成分及含量。不同提取部位在低浓度时促进细胞生长,高浓度时抑制细胞,不同提取部位活性顺序为SF-3>SF-4>SF-7>SF-6>SF-5>全方>SF-2>SF-1。结论少腹逐瘀汤不同提取部位成分及含量不同,不同提取部位活性有显著性差异。

Objective To determine the potential chemical compositions and their contents in the different isolated parts( numbered SF-1 to SF-7) of Shaofu Zhuyu Decoction,and observe their effects on eutopic endometrial cells in patients with endometriosis. Methods The samples of different parts( SF-1 to SF-7) were prepared by steam distillation,decoction,alcohol precipitation and D101 macroporous adsorption resin. The qualitative and quantitative analysis were performed by high performance liquid chromatography. The volatile oil components were analyzed by gas chromatography-mass spectrometry. The activity screening on each part of Shaofu Zhuyu Decoction was conducted through culturing the eutopic endometrial cells in patients with endometriosis. Results The different extraction parts of Shaofu Zhuyu Decoction were separated,and the main components and contents of different extraction parts were determined. The different extraction parts promoted the cell growth at low concentrations and inhibited the cells at high concentrations. The activity of different extraction parts were SF-3>SF-4>SF-7>SF-6>SF-5>whole decoction>SF-2>SF-1. Conclusion The different extraction parts of Shaofu Zhuyu Decoction show the different components and contents,and there are significant differences on the activity between the different extraction parts.