新型气升式反应器中浸没/非浸没周期对水母雪莲细胞培养的影响

Effect of Submerged/Unsubmerged Period on Cell Culture of Saussurea medusa in a Novel Air-lift Bioreactor

应用新型周期浸没气升式反应器,进行了水母雪莲细胞的浸没悬浮和非浸没静置交替循环培养,研究了培养过程中的关键条件—浸没/非浸没周期对水母雪莲细胞生长、黄酮合成及细胞聚集体调控的影响,发现5min/4h为最佳的循环周期。在此循环周期下,当通气量为40L/h、接种量(干重DW)为2～3g/L时,经20d的培养,细胞聚集体分布最佳,细胞干重达9.6g/L,黄酮类化合物的含量及黄酮类化合物的产量分别可达35.2mg/g(DW)及338mg/L。

The alternant submerged/unsubmerged cell culture of Saussurea medusa in a novel 1.8 L periodically submerged air-lift bioreactor was investigated. The bioreactor contains two layers of meshes. They provide support for cells in unsubmerged culture, but when being pulled slant the culture can be released by circulating medium from the meshes for submerged culture. The effects of the submerged/unsubmerged period on cell growth, biosynthesis of flavonoids and cell aggregate were studied. It was found that 5 min/4 h was the optimum submerged/unsubmerged period when the culture was carried out at air flow rate of 40 L/h and an inoculum (DW) of 2-3 g/L. After 20 d culture, the maximum biomass and production of flavonoids reached 9.6 g/L and 338 mg/L respectively, and the distribution of cell aggregates was the optimal as well.