玉米蛋白粉中黄体素和玉米黄素的分离与定性

Isolation and Identification of Lutein and Zeaxanthin from Corn Gluten by Thin-Layer Chromatography

利用溶剂萃取、薄层层析对玉米蛋白粉中黄体素和玉米黄素进行了提取和分离.通过薄层层析的相对迁移率、与AgNO3的显色反应、紫外 可见吸收光谱、高效液相色谱保留时间及液质联用所得到的相对分子质量信息,对分离所得黄体素和玉米黄素进行了定性研究,为玉米蛋白粉中黄体素和玉米黄素的进一步研究和开发利用奠定了基础.

Solventextraction and thinlayer chromatography were used to extract and separate lutein and zeaxanthin from corn gluten. Their structures were identified by comparing the Rf value with the standard, the color reaction with AgNO3, the UVspectrum, the retention time of RPHPLC, and the spectrum of LC/MS. The results provided the basic information for the further research.