脱盐过程中丝素蛋白缔合机理

Study on the Mechanism of Aggregation of Silk Fibroin in Desalting Process

采用透射电子显微镜(TEM)、X 射线衍射(X ray)、红外光谱(IR)、圆二色谱(CD)等测定了在脱盐过程中丝素蛋白分子构象的变化.随着丝素蛋白盐溶液脱盐率的增加,丝素蛋白分子从无规线团结构慢慢向β 折叠结构转变,导致丝素蛋白分子间发生缔合.

In this paper,the conformational transition of silk fibroin in desalting process was studied by transmission electron microscopy (TEM),Xray diffraction, Infrared (IR) spectroscopy,circular dichroism (CD). With the increase in the desalted rate, the conformation of silk fibroin changed from random coil to βsheet, leading to aggregation of silk fibroin.