草菇木聚糖酶SDS-PAGE后的复性及活性染色

Renaturation and Active Staining of Xylanase from Volvariella volvacea after SDS-PAGE

草菇木聚糖酶经SDS PAGE后在胶中能够原位复性,通过银染和刚果红活性染色比较,可确定该酶在胶中的位置.草菇木聚糖酶是单亚基蛋白,相对分子质量为24500.

Xylanase from Volvariella volvacea was shown to be renaturable after SDSPAGE. Enzyme activity was detected in situ by Congo red staining. Compared with silver staining and Congo red staining, xylanase could be located in gel. Xylanase from Volvariella volvacea was monomeric enzyme and its molecular weight was 24500.