摘要
草菇木聚糖酶经SDS PAGE后在胶中能够原位复性,通过银染和刚果红活性染色比较,可确定该酶在胶中的位置.草菇木聚糖酶是单亚基蛋白,相对分子质量为24500.
Xylanase from Volvariella volvacea was shown to be renaturable after SDSPAGE. Enzyme activity was detected in situ by Congo red staining. Compared with silver staining and Congo red staining, xylanase could be located in gel. Xylanase from Volvariella volvacea was monomeric enzyme and its molecular weight was 24500.
出处
《无锡轻工大学学报(食品与生物技术)》
CSCD
北大核心
2003年第5期102-103,110,共3页
Journal of Wuxi University of Light Industry