A study on the differences of effect on mast cells and serum 5-HT in the acupoint area of "CV 4" of rats by acupuncture stimulus and thermal stimulus

针刺和热刺激对小鼠“关元”穴区肥大细胞、血清五羟色胺影响差异的研究(英文)

Objective: To observe the influence of acupuncture stimulus and thermal stimulus on the expression of mast cells(MCs) and 5-hydroxytryptamine(5-HT) in local acupoint area of Guanyuan(关元 CV 4) and serum 5-HT in rats.Methods: Thirty-nine male C57 BL/6 rats were randomly divided into blank control group, acupuncture stimulus group and thermal stimulus group, with 13 rats in each group. The rats were stimulated by manual acupuncture at CV 4 for 5 min in acupuncture stimulus group, while those in thermal stimulus group were stimulated by adopting a thermal moxibustion apparatus on CV 4 for 30 min. The expression of MC and 5-HT in the skin in the acupoint area of CV 4 before and after acupuncture stimulus and thermal stimulus was observed and analyzed via adopting toluidine blue staining method and immunofluorescence histochemical method(5 rats were selected from each group), and the 5-HT content in serum before and after stimulus was determined through enzyme-linked immunosorbent assay(ELISA)(8 rats were selected from each group). The influence of acupuncture stimulus and thermal stimulus on the contents of MC and 5-HT in the skin and serum 5-HT in rats was analyzed and compared.Results: ① After acupuncture stimulus or thermal stimulus on CV 4 of C57 BL/6 rats, the number of MC in the acupoint area significantly increased when compared with that in blank group(the acupuncture stimulus group 12.40 ± 2.07 vs. the blank group 3.00 ±5.96;thermal stimulus group 26.20 ± 10.85 vs. the blank group 12.40 ± 2.07, both P< 0.05), and MC aggregation and degranulation were observed(the acupuncture stimulus group 17.80 ±4.55 vs. the blank group 8.00 ±3.16;the thermal stimulus group24.00±9.05 vs. the blank group 8.00±3.16. P<0.05, P< 0.01).② After acupuncture stimulus or thermal stimulus, 5-HT was released by MCs in the acupoint area, which aggregated around the blood vessels,and the number of 5-HT in the acupoint area significantly increased when compared with that in blank group(the blank group 3.00 ±1.28 vs. the acupuncture stimulus group 10.02 ±3.21;the blank group3.00 ±1.28 vs. the thermal stimulus group 14.00 ±3.94, both P< 0.01).③ Compared with blank group,both acupuncture stimulus and thermal stimulus could reduce the 5-HT content in serum(the blank group 0.72 ±0.2372 vs. acupuncture stimulus group 0.43 ±0.21: the blank group 0.72 ±0.24 vs. thermal stimulus group 0.32 ±0.18, both P<0.01), and the effect in thermal stimulus group was slightly superior to that in acupuncture stimulus group(P<0.05).Conclusion: Both acupuncture stimulus and thermal stimulus can cause the aggregation and degranulation of MCs and high expression of 5-HT in the acupoint area. The effect of thermal stimulus was superior to that of acupuncture stimulus in degranulation.

目的:观察针刺激与热刺激不同刺激方式对小鼠"关元"局部穴区肥大细胞(MC)、五羟色胺(5-HT)和血清中5-HT表达的影响。方法:39只雄性C57BL/6小鼠随机分为空白组、针刺激组、热刺激组,每组13只,适应性喂养一周后进行试验。针刺激组手针刺激小鼠"关元" 5 min,热刺激组采用热灸仪刺激"关元" 30 min。采甲苯胺蓝染色方法和免疫荧光组织化学方法观察分析针刺与热刺激前后对"关元"穴区皮肤MC和5-HT表达(每组5只),采用酶联免疫吸附测定法测定针和热刺激前后对血清中5-HT含量(每组8只),分析比较针刺激与热刺激对小鼠皮肤MC、5-HT以及血清5-HT含量的不同影响。结果:①针或热刺激C57BL/6小鼠"关元"穴后,其穴区肥大细胞数量较C57BL/6空白组明显增多(空白组12.40±2.07 vs针刺激组23.00±5.96,空白组12.40±2.07 vs热刺激组26.20±10.85,均P<0.05),出现肥大细胞聚集及脱颗粒现象(空白组8.00±3.16vs针刺激组17.80±4.55,空白组8.00±3.16 vs热刺激组24.00±9.05,P<0.05,P<0.01)。②针或热刺激后出现穴区肥大细胞释放5-HT,并向血管周围聚集,穴区5-HT数量较空白组相比明显增多(空白组3.00±1.28 vs针刺激组10.02±3.21:空白组3.00±1.28vs热刺激组14.00±3.94,均P<0.01)。③与空白组对比,针或热刺激均能降低血清中5-HT含量(空白组0.72±0.2372 vs针刺激组0.43±0.21,空白组0.72±0.24 vs热刺激组0.32±0.18,均P<0.01),而热刺激组略优于针组(P<0.05)。结论:针刺激、热刺激均可以引起穴区肥大细胞的聚集和脱颗粒以及5-HT高表达,热刺激的效用略优于针刺激。