EI注射液对鹅膏蕈氨酸基底核微量注射痴呆模型大鼠ATP/AMP及PI3K/AKT信号通路的影响

Effects of EI injection on Brain Energy and PI3K/AKT Signaling Pathway in IBO Basal Nucleus Microinjected with Dementia Model Rats

目的:考查EI注射液对双侧Meynert基底注射鹅膏蕈氨酸(Ibotenic acid,IBO)拟痴呆模型大鼠学习记忆功能及脑能量影响及机制。方法:采用双侧Meynert基底注射IBO建立拟痴呆模型大鼠,EI注射液干预8周,Morris水迷宫检测大鼠学习记忆能力,刚果红染色观察大鼠海马CA1区及皮质区Aβ斑块沉积的变化;HPLC测定各组动物脑组织ATP、ADP、AMP含量;ELISA试剂盒检测脑组织胰岛素含量;Western bolt法检测脑组织PI3K/AKT信号通路关键蛋白的表达。结果:与假手术组比较,模型组大鼠逃避潜伏期显著延长、进入平台次数、穿越平台象限时间及百分比明显减少(P <0.05);海马和皮层均出现明显红染的Aβ斑块沉积;脑组织ATP/AMP比率和胰岛素含量明显降低(P <0.05);脑组织PI3K和AKT蛋白呈低表达(P> 0.05)。经EI注射液干预后,该模型大鼠逃避潜伏期明显缩短、进入平台次数、穿越平台象限时间明显增加(P <0.05);海马和皮层红染减轻;脑组织ATP/AMP比率和胰岛素含量明显增加(P <0.05)。结论:EI注射液能够改善IBO拟痴呆模型大鼠学习记忆功能,该作用与改善脑能量有关。

Objective: To investigate the effects of EI injection on learning and memory ability and brain energy of two-way Meynert basal injection of Ibotenic acid(IBO) dementia model rats. Methods: A rat model of dementia wasestablished by bilateral meynert basal injection of IBO. After 8 weeks of EI injection, Morris water maze was used todetect the learning and memory ability of rats. Congo red staining was used to observe the deposition of Aβ plaque inhippocampal CA1 and cortical areas of rats. The changes of ATP, ADP and AMP in brain tissue of each group weredetermined by HPLC. The content of insulin in rat brain tissue was detected by ELISA kit. The expression of key proteinin PI3K/AKT signaling pathway was detected by Western blot. Results: Compared with the sham operation group, theescape latency of the model group was significantly prolonged, the number of entering the platform, the time andpercentage of crossing the platform quadrant decreased significantly(P < 0.05);Aβ plaque deposition was observed inthe hippocampus and cortex;ATP/AMP ratio and insulin content were significantly decreased(P < 0.05);brain tissue PI3 K and AKT protein were low expression(P > 0.05). After intervention with EI injection, the escape latency of themodel rats was significantly shortened, the number of entering the platform and the time of crossing the platform quadrantincreased significantly(P < 0.05);the hippocampus and cortex red staining was alleviated;the brain tissue ATP/AMPratio and insulin content increased significantly(P < 0.05). Conclusion: EI injection can improve the learning andmemory function of IBO-induced dementia model rats, which is related to the improvement of brain energy.