增强耐甲氧西林金黄色葡萄球菌抗生素敏感性的中药筛选

Screening of Traditional Chinese Medicine that Enhance the Sensitivity of Methicillin-resistant Staphylococcus aureus to Antibiotic

目的:研究中药提取物作用于耐甲氧西林金黄色葡萄球菌(MRSA)后,该菌株对苯唑西林敏感性的变化,旨在筛选能增强MRSA对抗生素敏感性的中药,为临床治疗MRSA感染提供新思路。方法:选取33种常用抗菌中药制备提取物,作用于临床分离的MRSA菌株,采取微量肉汤稀释法测定中药作用前后苯唑西林对MRSA的最低抑菌浓度(MIC),具有统计学差异(P <0.05)为具有增敏作用。结果:中药作用前,苯唑西林对MRSA的MIC为128-512μg·mL-1,白及醇提物作用后,MIC变化具有统计学差异(P <0.05),能够增加MRSA(271)对苯唑西林的敏感性。结论:白及醇提物能增强MRSA(271)对抗生素的敏感性,与抗生素配伍使用,有望恢复抗生素的杀菌效力。

Objective: To study the changes of the sensitivity of the methicillin-resistant Staphylococcus aureus(MRSA) to oxacillin after Chinese herbs. effects, so as to find Chinese medicine that can enhance the sensitivity of MRSA to antibiotics and provide new ideas for the clinical treatment of MRSA infection. Methods: A total of 33 commonly used antibacterial herbs were selected to prepare extracts, which act on clinically isolated MRSA. The broth micro dilution method was used to determine the minimal inhibitory concentration(MIC) of oxacillin on MRSA before and after the action of Chinese medicine. If there is a statistically significant difference(P < 0.05), the medicine is effective. Results:The MIC of oxacillin on MRSA were 128-512 μg·ml-1, after the effect of Bletilla extracts, the change of MIC showed a statistically significant difference(P < 0.05), which could increase the sensitivity of MRSA(271) to oxacillin. Conclusion:Bletilla extracts can enhance the sensitivity of MRSA(271) to oxacillin and the compatibility with antibiotics is expected to restore the efficacy of antibiotic.