用荧光法快速检测苯并(a)芘对人外周血白细胞DNA的损伤作用

Fluorometric Method For In-vitro Rapid Detection of DNA Damage to Human Peripheral Leukocytes Induced by Benzo(a)pyrene

采用荧光快速检测 DNA 解螺旋(FADU)方法检测了环境致癌物苯并(a)芘在体外对人外周血白细胞 DNA 的损伤作用。结果显示,经大鼠肝 S_■混合液活化的苯并(a)芘在0.01～100.0μM 剂量范围内可使白细胞 DNA 链断裂明显增加,细胞内剩余双链 DNA 降低24～61%(P<0.05),且存在明显的剂量效应关系。未经活化的苯并(a)芘则未出现明显的 DNA 损伤效应。结果还提示,苯并(a)芘的 DNA 损伤效应是由其活性代谢产物引起的,该 FADU 检测方法同(?)适用于检测能引起 DNA 链断裂的间接致突变物和致癌物。

A preliminary study on application of Fluorometric analy- sis of DNA unwinding(FADU)in the detection of DNA strand breaks indu- ced by indirect environmental mutag- en benzo(a) pyrene[BaP]was condu- cted.The results showed that BaP, activated by PCB-induced rat liver S(?) mixture,could result in obvious inc- rease of DNA strand breaks in human peripheral leukocytes incubated with BaP(0.01,0.1,1.0,10.0,and 100.0μM) for 60 min under 37℃ condition;co- mpared to DMSO solvent control,the residual DNA double strands in leuk- ocytes were significantly decreased (P<0.05)and existed a clear dose re- sponse relationship,but these chang- es were not present when inactivated BaP(10.0 and 100.0μM)were used.The results suggested that the effect of DNA strand breaks induced by BaP was caused by the latter's active metabolites;and,FADU is also suita- ble for the detection of DNA strand breaks caused by indirect mutagens and carcinogens.