摘要
Objective: To assess impact of probiotics and breastfeeding on gut microecolog y. Study design: Mothers were randomized to receive placebo or Lactobacillus rha mnosus GG before delivery, with treatment of the infants after delivery. We asse ssed gut microbiota, humoral immune responses, and measured soluble cluster of d ifferentiation 14 (sCD14) in colostrumin 96 infants. Results: Fecal Bifidobacter ium and Lactobacillus/Enterococcus counts were higher in breastfed than formula-f ed infants at 6 months; P < .0001 and P = .01, respectively. At 3 months, total number of immunoglobulin (Ig)G-secreting cells in breastfed infants supplemente d with probiotics exceeded those in breastfed infants receiving placebo; P = .05 , and their number correlated with concentration of sCD14 in colostrum. Total nu mbers of IgM-, IgA-, and IgG-secreting cells at 12 months were higher in infa nts breastfed exclusively for at least for 3 months and supplemented with probio tics as compared with breastfed infants receiving placebo; P = .005, P = .03 and P = .04, respectively. Again, sCD14 in colostrum correlated with numbers of IgM and IgA cells; P = .05 in both. Conclusions: We found an interaction between pr obiotics and breastfeeding on number of Ig-secreting cells, suggesting that pro biotics during breastfeeding may positively influence gut immunity.
Objective: To assess impact of probiotics and breastfeeding on gut microecolog y. Study design: Mothers were randomized to receive placebo or Lactobacillus rha mnosus GG before delivery, with treatment of the infants after delivery. We asse ssed gut microbiota, humoral immune responses, and measured soluble cluster of d ifferentiation 14 (sCD14) in colostrumin 96 infants. Results: Fecal Bifidobacter ium and Lactobacillus/Enterococcus counts were higher in breastfed than formula-f ed infants at 6 months; P < .0001 and P = .01, respectively. At 3 months, total number of immunoglobulin (Ig)G-secreting cells in breastfed infants supplemente d with probiotics exceeded those in breastfed infants receiving placebo; P = .05 , and their number correlated with concentration of sCD14 in colostrum. Total nu mbers of IgM-, IgA-, and IgG-secreting cells at 12 months were higher in infa nts breastfed exclusively for at least for 3 months and supplemented with probio tics as compared with breastfed infants receiving placebo; P = .005, P = .03 and P = .04, respectively. Again, sCD14 in colostrum correlated with numbers of IgM and IgA cells; P = .05 in both. Conclusions: We found an interaction between pr obiotics and breastfeeding on number of Ig-secreting cells, suggesting that pro biotics during breastfeeding may positively influence gut immunity.
