黄龙汤对脓毒症大鼠内毒素及降钙素原的影响

Impacts of Huanglong Decoction on Endotoxin and Procalcitonin in the Rats of Sepsis

目的探讨黄龙汤对脓毒症大鼠血清内毒素及降钙素原的影响。方法将55只wistar大鼠随机分为假手术组、模型对照组、西药组和中西药联合治疗组,模型对照组、西药组和中西药联合治疗组大鼠建立腹腔感染脓毒症致肠黏膜屏障损害大鼠模型。模型复制成功后,假手术组、模型对照组按2 ml/次的剂量灌胃给予生理盐水,西药组大鼠每天给予美罗培南50 mg/(次·只),日2次腹腔注射;中西药联合治疗组大鼠每日给予美罗培南50 mg/(次·只),日2次腹腔注射,同时给予黄龙汤煎剂经口灌喂,每日2次。于造模后4 d腹主动脉取血检测。结果模型组大鼠外周血内毒素及降钙素原较假手术组显著升高(P<0.01)。西药组和中西药联合治疗组比模型组明显降低(P<0.01),中西药联合治疗组低于西药组(P<0.05,P<0.01)。结论中药黄龙汤联合美罗培南治疗肠源性脓毒症,能有效降低血清内毒素、降钙素原的含量及血液中的炎症介质浓度,缓解机体过度的炎症反应。

Objective To explore the impacts of huanglong decoction on endotoxin and procalcitonin in the rats of sepsis. Methods Fifty- five Wistar rats were randomized into a sham- operation group,a model control group,a western medicine group and an integrated Chinese and western medicine group. In the model control group,western medicine group and treatment group,the model of intestinal mucosal barrier induced by sepsis of abdominal infection was established. After successful duplication of model,the physical saline was used for gastric perfusion at 2 ml / time in the sham- operation group and the model control group.The intraperitoneal injection of meropenem,50 mg / time,twice a day was applied in the western medicine group every day. In the integrated Chinese and western medicine group,the intraperitoneal injection of meropenem was applied,50 mg / time,twice a day; additionally,huanglong decoction was fed,twice a day. In 4 days of modeling,blood was collected via abdominal aorta for detection. Results The endotoxin and procalcitonin in peripheral blood in the model group were increased significantly as compared with the sham- operation group( P < 0. 01). Those in the western medicine group and the integrated Chinese and western medicine group were lower apparently than those in the model group( P < 0. 01). The levels of endotoxin and procalcitonin in the integrated Chinese and western medicine group were lower than those in the western medicine group( P < 0. 05,P < 0. 01). Conclusion The combined medication of huanglong decoction and meropenem reduces the levels of plasma endotoxin and procalcitonin,effectively decreases the concentration of inflammatory medium in blood and relieves excessive inflammatory reaction in the treatment of enterogenous sepsis.