定向透药离子导入联合骨伤膏对骨折的影响

Impacts on Bone Fracture Treated with Directional Medicine Iontophoresis and Guoshang Ointment

目的探讨定向透药离子导入联合骨伤膏对骨折愈合的影响,明确该项联用技术是否有加强骨折愈合的作用及可能的机制。方法将雄性健康新西兰兔80只随机等分为定向透药离子导入联合骨伤膏给药组(给药组)和仅给予骨伤膏治疗的骨伤膏对照组(对照组),每组40只,均建立右侧桡骨骨折模型,以第2、4、6、8周4个时间点将每组动物随机等分成4组,每组10只。给药组所有动物右侧骨折部位给予定向透药离子导入联合骨伤膏治疗,对照组右侧骨折部位单纯给予骨伤膏治疗。术后两大组动物在治疗后的第2、4、6、8周4个时间点进行血清碱性磷酸酶(AKP)活性及骨钙素(BGP)含量测定,骨密度和骨矿物质含量,X线影像学检查,组织切片和形态学检测。结果与对照组比较,给药组治疗的第2周,血清AKP活性水平及BGP含量较对照组显著升高,差异有统计学意义(P<0.05),随着时间的推移,第4、6、8周,与对照组比较,给药组变化愈加明显,其中第6、8周尤为突出(P<0.01)。另外,与对照组比较,骨密度、骨矿物质含量、X线影像学半定量,组织切片和形态学检测亦显示给药组骨折愈合明显提前,各时间点两组间比较,差异均有统计学意义(P<0.05,P<0.01)。结论定向透药离子导入联合骨伤膏通过对骨折愈合的整个过程施加影响从而加速了骨折的愈合。

Objective To discuss the impacts on the healing of bone fracture treated with direction-al medicine iontophoresis combined with gushang ointment and determine whether this combined therapy could enhance the effect of healing in bone fracture and discover the potential mechanism. Methods The male healthy New Zealand 80 rabbits were randomized into a group of directional medicine iontophoresis com-bined with gushang ointment(medication group)and a control group with gushang ointment(control group), 40 cases in each one. The model of right radial fracture was set up. At 4 time points,named in the 2nd ,4th ,6th and 8th weeks,the animals in each group were randomized into 4 subgroups separately,10 cases in each one. In the medication group,the combined therapy of directional medicine iontophoresis combined with gushang ointment was applied to the affected region and in the control group,only gushang ointment was used. At 4 time points,named in the 2nd ,4th ,6th and 8th weeks,the serum alkaline phosphatase(AKP)activity,the con-tent of bone gla protein(BGP),bone density and bone mineral density were determined. X - ray imaging was used for the examination. The tissue slices and morphologic test were conducted. Results Compared with the control group,in the 2nd week,serum AKP activity and BGP content in the medication group were increased significantly as compared with the control group,indicating the significant difference(P < 0. 05). With the time going,in the 4th ,6th and 8th weeks,compared with the control group,the changes in the medication group were getting more obvious,especially in the 6th and 8th groups(P < 0. 01). Additionally,compared with the control group,the bone density,bone mineral density,semiquantitative in X - ray imaging as well as the de-tection of tissue slice and morphology all indicated that bone fracture was healed apparently in advance in the medication group. The differences were significant in comparison between the two groups at each time point(P< 0. 05,P < 0. 01). Conclusion The directional medicine iontophoresis combined with gushang ointment impacts the entire healing process so as to accelerate the healing in bone fracture.