四君子汤对脾虚小鼠感染念珠菌的致病性研究

Study of Sijunzi Tang on the Pathogenicity due to Candia Infection in the Mice of Spleen Deficiency

目的研究脾虚时经口感染白念珠菌后经四君子汤灌胃治疗后机体免疫力的变化情况。方法将50只小鼠随机分为对照组(A组)、脾虚模型组(B组)、脾虚+白念珠菌组(C组)、脾虚+白念珠菌+氟康唑组(D组)、脾虚+白念珠菌+四君子汤组(E组),每组10只。除A组外,其余组别采取复合因素制造脾虚小鼠模型,经口感染白念珠菌,测定小鼠肾脏内和粪便中活菌菌数,观察脾虚小鼠的肠黏膜在光镜下的形态改变。结果与A组相比,B组小鼠粪便中的活菌菌数明显增多(P<0.05),小肠上皮的一些微绒毛、细胞器等结构改变比较明显。C组与E组小鼠比较,E组肾脏以及粪便中的活菌菌数明显减少(P<0.01),小肠上皮绒毛等结构有所恢复,肠腺排列整齐,黏膜下层结构基本完整,未见充血及水肿等现象。结论脾虚经口感染白念珠菌后免疫力下降,而四君子汤可以益气健脾,提高机体免疫力,从而达到治疗作用。

Objective To study the changes of body immunity after the lavage of sijunzi tang in the mice after candida infection at the condition of spleen deficiency. Methods Fifty mice were randomized into a control group( A group),a spleen- deficiency model group( B group),a spleen deficiency + candida group( C group),a spleen deficiency + candida + fluconazole( D group) and a spleen deficiency + candida + sijunzi tang group( E group),10 mice in each one. Except the A group,the compound factors were adopted to prepare spleen deficiency mice models in the rest groups. After candida infection,the viable bacterial count in the kidney and stool was determined and the morphologic changes of intestinal mucosa were observed under light microscope. Results As compared with the A group,the viable bacterial count in stool was increased apparently in the B group( P < 0. 05) and the structural changes in some villus and organelle of the small intestine epithelium were more apparent. In comparison between the C and E groups,the viable bacterial count in the kidney and stool was reduced apparently( P < 0. 01),the structure of villus of small intestine epithelium was recovered,the enteraden was well arranged,submucous structure was complete basically and the congestion and edema were not found. Conclusion The immunity is reduced after candida infection in the mice of spleen deficiency. Sijunzi tang benefits qi,strengthens the spleen and improves the body immunity so that to treat the disease.