补肾中药促进兔前交叉韧带重建术后腱—骨愈合的组织学观察

Histological Study of Herbal Medicine for Kidney Tonification on Tendon-Bone Healing after Anterior Cruciate Ligament Reconstruction in Rabbits

目的观察中药干预兔前交叉韧带重建术后腱—骨愈合的组织学变化,探讨中药对腱—骨愈合的促进作用。方法将36只新西兰大白兔随机分为温补肾阳组、滋补肾阴组和空白对照组三组,每组12只。构建兔前交叉韧带重建术后模型,使用中药干预并制备组织标本,光镜下观察腱—骨愈合情况,评价各组腱—骨界面的组织学变化。结果 HE染色显示滋补肾阴组的腱—骨界面区内成纤维细胞、软骨细胞的生长情况优于温补肾阳组,温补肾阳组优于空白对照组。Masson染色显示滋补肾阴组的胶原纤维增生情况最优,温补肾阳组次之,空白对照组的胶原纤维增生最慢。结论补肾中药能促进前交叉韧带重建术后的腱—骨愈合,但其具体作用机制尚需进一步的研究加以阐释。

Objective To observe the histological changes in tendon- bone healing after anterior cruciate ligament( ACL) reconstruction in the intervention of herbal medicine in the rabbits so as to explore the enhancing effect of herbal medicine on tendon- bone healing. Methods Thirty- six New Zealand rabbits were randomly divided into a kidney yang tonification group,a kidney yin nourishment group and a blank control group,12 rabbits in each one. The models of ACL reconstruction were prepared. After the intervention of herbal medicine,the histological specimen was collected. The tendon- bone healing condition was observed under optic microscope. The histological changes on the interface of tendon and bone were assessed.Results HE staining indicated that the growth of fibrocytes and chondracyes on tendon- bone interface in the kidney yin nourishment group was better than that in the kidney yang tonification and the result in the kidney yang tonification group was better than that in the blank control group. Masson staining showed that fibrocytes was proliferated the best in the kidney yin nourishment group and the proliferation in the kidney yang tonification was slower and that in the blank control group was the slowest. Conclusion The herbal medicine for kidney tonification promotes tendon- bone healing after ACL reconstruction. It is expected to have a study on this mechanism for further explanation.