天蓝淡红链霉菌发酵生产柔红霉素的研究

Study on Production of Daunorubicin Treptomyces coeruleorubidus Fermentation

为了提高柔红霉素发酵的产量,减少生产成本和减轻患者负担。采用正交实验设计方法对天蓝淡红链霉菌ST511发酵生产柔红霉素的发酵工艺进行了优化。首先,采用单因素实验确定生产柔红霉素的培养基组成成分和发酵条件,然后进行正交实验,得到最优的培养基组合:葡萄糖50g/L、酵母粉65g/L、Ca CO34g/L、Fe SO4·7H2O 0.7g/L,最优的发酵工艺为:初始p H 6.9、种龄48h、发酵时间7d、发酵温度28℃。在此条件下摇瓶发酵产量为2.86g/L,提高了12%;于50L和1000L发酵罐产量为2.88g/L、2.85g/L,提高了15%、17%。

In order to improve daunorubicin fermentation production, reduce production costs and reduce the burden of patients. By using the orthogonal design method of fermentation process for producing daunorubicin fermentation of Streptomyces coeruleorubidus ST511 were optimized. First of all, using the single factor experiment to determine the medium for the production of daunorubicin composition and fermentation conditions, and then the orthogonal experiment, the optimal culture medium was: glucose 50g/L, yeast 65g/L, Ca CO34g/L, Fe SO4·7H2O 0.7g/L, the optimal fermentation process: initial p H 6.9, seed age 48 h, fermentation time 7d, fermentation the temperature of 28℃.Under the condition of shaking flask fermentation yield was 2.86g/L, increased by 12%; 50 L and 1000 L fermentation yield was 2.88g/L, 2.85g/L, increased by 15%, 17%.