摘要
阳离子基因载体表面带有大量正电荷,由于许多DNA和细胞膜表面带负电荷,因此阳离子基因载体表面电荷有利于提高结合DNA的效率,纳米粒子与细胞膜的吸附也受粒子表面电荷的影响。同时,其表面电荷也是产生细胞毒性的主要原因之一,因此揭示细胞毒性及其作用机制有利于开发出更安全高效的基因递送载体。本文综述了阳离子基因载体表面电荷对DNA结合能力、细胞摄入、转染效率以及细胞毒性及其作用机制的影响。
The surface of the cationic gene vectors carries a large amount of positive charges.Because many DNA and cell membrane surfaces are negatively charged,the positive charges on the surface of the cationic vectors are beneficial to increase the efficiency of DNA loading.The adsorption of nanoparticles with cell membrane is also affected by the surface charge of the particles.At the same time,its surface positive charge is also the main cause of cytotoxicity,so revealing the mechanism will help to develop much safer and efficient gene delivery vectors.This paper reviews the effect of the positive charge on the surface of cationic nanocarriers with DNA binding capacity,cellular uptake,transfection efficiency,and cytotoxicity.
作者
付莹
崔韶晖
韩磊
王玥莹
马启晨
张树彪
FU Ying;CUI Shaohui;HAN Lei;WANG Yueying;MA Qichen;ZHANG Shubiao(College of Bioengineering,Dalian Polytechnic University,Dalian 116034,China;College of Life Sciences,Key Laboratory of Biotechnology and Bioresources Utilization,Ministry of Education,Dalian Minzu University,Dalian 116601,China)
出处
《生命的化学》
CAS
CSCD
2019年第4期744-752,共9页
Chemistry of Life
基金
国家自然科学基金项目(21776044)
中央高校自主科研基金项目(DC110177)
关键词
表面电荷
DNA结合
细胞摄入
转染效率
细胞毒性
surface charge
DNA binding
cellular uptake
transfection efficiency
cytotoxicity
