携带“F-5”基因片段人脐带间充质干细胞治疗大鼠皮瓣缺血再灌注损伤的研究

Study of the use of human umbilical cord mesenchymal stem cells carrying the“F-5”gene fragment in the treatment of abdominal flap ischemia-reperfusion injury in rats

目的通过动物实验证实,携带热休克蛋白90α(Hsp90α)功能片段的人脐带间充质干细胞(hUC-MSCs)能够有效改善大鼠腹部皮瓣缺血再灌注损伤。方法本实验将携带"F-5"基因的hUC-MSCs通过点状注射的方式应用于大鼠腹部皮瓣缺血再灌注损伤模型。将96只大鼠随机分为4组,即携带"F-5"基因组、空载病毒组、未转染组和未注射处理组。观察不同时间段各组模型皮瓣的坏死面积、颜色、毛发生长及皮瓣血管周边CD31的含量。结果携带"F-5"基因组皮瓣颜色和毛发生长情况均优于其他实验组,皮瓣坏死面积明显降低。免疫组化结果显示,携带"F-5"基因组皮瓣血管周边CD31含量远高于空载病毒组、未转染组和未注射处理组。结论携带"F-5"基因片段的hUC-MSCs能有效改善皮瓣缺血再灌注损伤。

Objective To confirm that human umbilical cord mesenchymal stem cells(h UC-MSCs)carrying the functional fragment of heat shock protein 90α(Hsp90α)can effectively improve ischemia-reperfusion injuries of abdominal flaps in rats.Methods In this study,h UC-MSCs carrying the'F-5'gene were applied to the abdominal flap ischemia-reperfusion injury model in rats through punctate injection.Ninety-six rats were randomly divided into four groups:(1)rats to be injected with h UC-MSCs transfected by adenovirus carrying the'F-5'gene;(2)rats to be injected with h UC-MSCs transfected by'non-carrying'adenovirus;(3)rats to be injected with pure h UC-MSCs without transfection;and(4)the control group(rats without injection).The necrosis area,color,hair growth and CD31 level around the flap blood vessels of each group were observed at various times.Results The flap color and hair growth of the group carrying the'F-5'gene were better than the other groups,and the necrosis area of the flap was significantly reduced.The result of immunohistochemistry showed that the CD31 level around the flap blood vessels of the group carrying the'F-5'gene was much higher than those of the group transfected without the'F-5',the non-transfection group,and the control group.Conclusion This study suggested that h UC-MSCs carrying the'F-5'gene fragment effectively improved flap ischemia-reperfusion injuries.