微渗析-液相色谱电化学检测法测定全血中的半胱氨酸和谷胱甘肽

Determination of cysteine and glutahione in human whole blood by combination of microdialysis sampling technique with liquid chromatography by electrochemical detection

采用微渗析取样作为样品预处理技术,与高效液相色谱电化学检测联用,建立了一种测定血中半胱氨酸(Cys)和谷胱甘肽(GSH)的新方法。电化学检测以玻碳电极为工作电极,结果表明在3.5×10-6～1.0×10-3mol/L浓度范围内,Cys和GSH的浓度分别与氧化峰的峰电流呈良好的线性关系,线性相关系数分别为0.9971和0.9982,检测限分别为1.2×10-6mol/L和3.2×10-6mol/L。

A method to determine cysteine(Cys) and glutathione(GSH) in human blood by combination of microdialysis sampling technique with high performance liquid chromatography by electrochemical detection was proposed. A glass carbon electrode was used as working electrode for detection. The results indicated that the peak currents of Cys and GSH were linear to their concentrations ranging from 3.5×10-6 to 1.0×10-3 mol/L, and the correlation coefficients were 0.9971 and 09982 with the detection limits of 1.2×10-6 and 3.2×10-6 mol/L, respectively.