减量训练通过不同亚型的PI3K对心肌的影响机制

The Mechanism of Tapering Influencing Cardiac Muscles Through PI3K of Different Subtypes

目的:探讨在减量训练过程中,不同亚型的PI3K影响心肌的机制。方法:72只雄性SD大鼠随机分为:3周安静对照组(C3)和3周大运动量训练组(E3);3天对照组(C4)和3天减量训练组(E4);6天对照组(C5)和6天减量训练组(E5);2周对照组(C6)和2周减量训练组(E6)。3周大运动量训练和2周的减量训练后取左心室肌组织,测定PI3K p85、PI3K p110α和PI3K p110γ蛋白采用Western blotting方法。心肌组织形态学采用HE染色在光镜下观察。结果:3周大运动量训练和2周减量训练后,心肌组织形态没有显著变化,未发现心肌组织损伤。6天减量训练后,心肌PI3K p110α蛋白表达显著增加(P<0.05)。2周减量训练后,心肌PI3K p110α/PI3K p85显著增加(P<0.05)。3周大运动量训练和减量训练后,心肌PI3K p110γ蛋白表达没有显著变化(P>0.05)。结论:本研究制定的运动训练和减量训练方案未造成心肌损伤。减量训练通过不同亚型的PI3K影响心肌。

Objective: To explore the cardiac muscle be affected by PI3 K in the course of tapering and its mechanism.Methods: 72 male SD rats were randomly divided into 8 groups after one week of adaptability training: the group that rest for 3 weeks( C3),the group that receive large amount of training for 3 weeks( E3); the group that rest for 3 days( C4) and the group that receive 3 days of tapering( E4); the group that rest for 6 days( C5) and the group that receive 6 days of tapering( E5); the group that rest for 2 weeks( C6) and the group that receive2 weeks of tapering( E6). After 3 weeks of large amount of training and 2 weeks of tapering,the cardiac muscle of left ventricle was sampled. PI3 K p85,PI3 K p110α and PI3 K p110γ protein was tested through Western blotting. The morphological structure of cardiac muscle organization was HE colored and observed under light microscope. Results: After 3 weeks of large amount of training and 2 weeks of tapering,no significant change occurred in the conformation of cardiac muscle organizations. No injuries were observed in the cardiac muscles.PI3 K p110α protein expression increased significantly in cardiac muscles after 6 days of tapering( P < 0. 05).PI3 K p110γ didn't change significantly after 3 weeks of large amount of training and after tapering( P >0. 05). Conclusions: The training and tapering in the project didn't result in any injuries of the cardiac muscles. Cardiac muscles can be affected by tapering through PI3 K.