副溶血弧菌PCR检测方法研究进展

Research progress on polymerase chain reaction detection method for Vibrio parahaemolyticus

副溶血弧菌是引起包括我国在内的世界各地沿海地区食物中毒的重要食源性致病菌,患者有典型的肠胃炎症状。及时准确地对食品中的副溶血弧菌进行检测是预防该菌引起的食物中毒的关键。分子生物学检测方法在副溶血弧菌检测中具有许多优势,现已得到广泛的应用。本文对PCR检测方法中的多重PCR、有扩增内标的PCR、实时荧光PCR(包括荧光染料法和荧光探针法)、基于DNA染料叠氮溴化乙锭和叠氮溴化丙锭的PCR、纳米粒子PCR、免疫捕获PCR、PCR-变性高效液相色谱、PCR-酶联免疫吸附等方法的国内外研究情况进行了综述,并对其检测效率、灵敏度、优点和缺点等进行了分析比较,环介导等温扩增(loop-mediated isothermal amplification,LAMP)——包括常规LAMP和原位LAMP因与PCR方法有相似之处,故一并进行了综述,为副溶血弧菌PCR检测方法的应用与开发提供参考。

Vibrio paraheamolyticus is a leading cause of foodborn disease in the littoral areas worldwide, including China. The key to prevent foodborn disease caused by V. paraheamolyticus is rapid and accurate de-tection of this bacterium. The molecular biology method is commonly used now for its abundant advantages. The recent research progress both domestic and overseas was summarized in this paper and the detecting effi-ciency, sensitivity and the relative merits among multiplex PCR, internal amplification control PCR, real-time PCR, ethidium monoazide bromide PCR or propidium monoazide PCR, nanoparticle-based assisted PCR, im-munocapture PCR, PCR-denaturing high performance liquid chromatography and PCR-enzyme-linked immu-nosorbent assay were also compared and analyzed for the purpose of providing reference for the further re-searches and development. Loop-mediated isothermal amplification (LAMP), including conventional LAMP and in situ LAMP, similar to PCR, also is summarized.