黄曲霉毒素新型抗体制备研究进展

Research progress of aflatoxin B_1 antibody preparation

免疫学检测方法具有快捷、灵敏、特异性高的特点,在毒素的定量和定性方面已获得了快速的发展,成为毒素检测方法的研究热点。而高质量抗体的制备是建立特异性强、灵敏度高的免疫分析方法关键。目前主流研究和应用的抗体是单克隆抗体,其性质稳定,特异性强,灵敏度高。随着抗体技术的发展,重组抗体在免疫检测领域也逐渐应用。与多克隆抗体、单克隆抗体相比较而言,重组抗体具有独特的优势,可以在原核表达体系里短时间内大量生产且生产费用低廉,对黄曲霉毒素的低成本、大规模检测有重要的应用价值。本文重点阐述和分析了黄曲霉毒素单克隆抗体、重组抗体制备过程中存在的影响因素及问题,并对未来黄曲霉毒素抗体的发展前景进行了展望。

Immunological detection methods with fast, sensitive and highly specific characteristics had gained a rapid development and become a hot detection methods. High quality antibody was the key of aflatoxins determination by specific and sensitive immunoassay methods. Monoclonal antibody was the most popular antibody in current researches and commercial products for their stability, high specificity and high sensitivity. More and more recombinant antibodies were researched and applied with the development of antibody technology. Compared to these two types of antibodies, an abundance of recombinant antibody can be produced in prokaryotic expression systems in a very short time with low cost. It will be helpful to the low-cost and high throughput detection method of aflatoxins. In this paper, the preparation processing of monoclonal and recombinant antibodies in aflatoxin detection were summarized and the key factors in the preparation of aflatoxins antibody were analyzed.