反相高效液相色谱法同时检测不同样品中6种五味子木脂素

Simultaneous determination of six lignans in Schisandra chinensis from different samples by reverse phase high-performance liquid chromatography

目的建立反相高效液相色谱法同时测定五味子中6种木脂素含量的方法,并应用此方法检测南、北五味子及3种市售成药:参芪五味子片、安神补心片、安神补心胶囊中此6种木脂素的含量。方法采用SHIMADZU VP-ODS(4.6 mm×150 mm i.d,5μm)色谱柱,以乙腈-水为流动相,进行梯度洗脱,流速0.5mL/min,检测波长220 nm,柱温30℃。结果五味子醇甲、五味子醇乙、五味子酯甲、五味子甲素、五味子乙素和五味子丙素分离度良好,分别在0.243~2.430μg、0.087~0.870μg、0.060~0.600μg、0.093~0.930μg、0.246~2.460μg、0.063~0.630μg的进样量范围内有良好的线性关系,平均加样回收率分别为100.93%、100.59%、99.78%、100.43%、96.72%、102.33%,RSD值分别为2.14%、2.95%、2.26%、2.81%、1.88%、0.985%。结论本研究所建立的反相高效液相色谱法方法准确可靠、简单可行,可用于五味子中木脂素类成分的定量分析,为五味子药材及以五味子为组方药味的中成药的质量评价奠定基础。

Objective To establish a method for simultaneous determination of six lignans in Schisandra chinensis by reverse phase high performance liquid chromatography (RP-HPLC), and apply this method to detect the content of 6 lignans in Kadsurasinensis, Schisandra chinensis and 3 commercially available patent medicine:Shenqi wuweizi tablets, Anshen buxin tablets, Anshen buxin capsules. Methods The analysis was performed on a SHIMADZU VP-ODS (4.6 mm×150 mm i.d., 5 μm) chromatographic column, and used acetonitrile-water as gradient elution mobile phase, the flow rate was 0.5 mL/min, the detection of wavelength was 220 nm and the column temperature was 30 ℃. Results The method showed an excellent chromatographic separation for the 6 lignans, and showed a good linearity as the injection volume of Schisandrol A, Schisandrol B, Schisantherin A, Schizandrin A, Schizandrin B, and Schizandrin C in 0.243~2.430 μg, 0.087~0.870 μg, 0.060~0.600 μg, 0.093~0.930 μg, 0.246~2.460 μg, and 0.063~0.630 μg. At the same time, the average extraction recoveries were 100.93%, 100.59%, 99.78%, 100.43%, 96.72%, and 102.33%, respectively; the RSD were 2.14% , 2.95%, 2.26%, 2.81% , 1.88% , and 0.985%, respectively. Conclusion The RP-HPLC method established in this study was accurate and reliable, simple and feasible, which could be used to analyze the lignans quantitatively and laid the foundation for the quality evaluation of Schisandra chinensis.