不同提取方法对蓝莓色素得率及抗氧化活力的影响研究

Effects of extraction methods on yield and antioxidant capacity of blueberry pigments

目的研究不同提取方法对蓝莓色素得率、抗氧化活力的影响及得率与抗氧化活力间的关系。方法分别以乙醇、甲醇、丙酮为浸提溶剂,常规溶剂浸提、超声辅助浸提、微波辅助浸提3种提取方式,测定浸提液色素得率及其清除DPPH·、·O·_2^-、·OH的能力;分析色素得率与抗氧化活力的关系。结果不同提取溶剂对蓝莓色素得率的影响差异不显著(P>0.05),但不同提取方式对蓝莓色素得率的影响差异显著(P<0.05);不同提取方法的蓝莓色素清除DPPH·、·O·_2^-、和·OH的能力存在显著差异(P<0.05);蓝莓色素得率与其清除DPPH·与·OH能力间存在显著的正相关。结论提取方式对蓝莓色素的得率及抗氧化活力有显著影响;蓝莓色素得率与其清除DPPH·和·OH能力呈显著的正相关性;超声辅助乙醇浸提更有助于获取高得率、高抗氧化活性的蓝莓色素。

Objective To evaluate the effects of different extraction methods on yield,antioxidant activity and the relationship between the yield and antioxidant activity of blueberry pigments.Methods The blueberry pigments were extracted by conventional solvent extraction,ultrasound-assisted extraction or microwave-assisted extraction with ethanol,methanol or acetone as solvent extraction and its yields and scavenging capacities to DPPH·,·O·_^-and.OH were determined.Results The yields of blueberry pigments extracted with ethanol,methanol or acetone were very similar(P>0.05),but the yields and scavenging capacities to DPPH·,·O·_2^-and.OH of blueberry pigments extracted by conventional solvent extraction,ultrasound-assisted extraction or microwave-assisted extraction were significantly different(P<0.05).There were significant positive correlations between the yields and scavenging capacities to DPPH·and ·OH of blueberry pigments,respectively(R=0.793,0.944;P<0.05).Conclusion The yields and scavenging capacities of blueberry pigments obtained by different extraction methods with the same extraction solvent are significantly different.The yields are significantly correlated with scavenging capacities of DPPH·and ·OH.It will help to get blueberry pigments with higher yield and higher antioxidant activity by ultrasonic assisted ethanol extraction.