砷含量测定在蜂蜜掺假鉴别中的应用

Arsenic detection in the application of honey adulteration

目的建立一种通过检测蜂蜜中砷的含量来鉴别大米糖浆掺假的方法。方法检测并比较大量不同来源的大米糖浆和纯正蜂蜜中砷的含量,同时优化了蜂蜜中砷的检测方法,样品以硝酸-微波消解,电感耦合等离子体质谱(ICP-MS)测定,内标法定量。结果在0~5.0μg/L范围内线性良好,定量限为10μg/kg,10、15和30μg/kg的添加水平的回收率均在83.7%~109.6%之间,相对标准偏差小于5.9%,并确定了蜂蜜中大米糖浆的阳性判定限,砷含量在15μg/kg以上的蜂蜜为大米糖浆掺假蜂蜜。结论该方法前处理简单,结果准确度和精密度高,纯蜂蜜和大米糖浆的中砷的含量差别明显,可用于蜂蜜中大米糖浆的掺假鉴别。

Objective To develop a method for detecting the arsenic concentration in honey to identify rice syrup adulteration.Methods Different sources and numbers of honeys and rice syrup were detected and compared by arsenic concentration. The experimental conditions for the arsenic determination in honey by mi-crowave digestion/inductively coupled plasma mass spectrometry (ICP-MS) were optimized.Results Good linearity was obtained in the range of 0~5μg/L for arsenic detection with the quantitation limit of 10μg/kg. The recovery ranged from83.7%~109.6% at three levels of 10, 15 and 30μg/kg with relative standard deviation less than 5.9%. The concentration limit of positive rice syrup in honey was determined. The arsenic concentration over 15μg/kg was identified as the rice syrup adulteration honey.Conclusion It has significant difference between pure honey and adulterated honey. The method is fast, easy and suitable for identifying rice syrup in honey adulteration.