基于SPME-GC-MS的四季桂花和枝叶挥发性物质测定

Analysis of the volatile components from flowers and leaves of Osmanthus fragrans var.semperflorens by GC-MS combined with SPME

目的研究四季桂花的香气及枝叶的挥发性物质组成。方法采用固相微萃取法富集四季桂花的香气及枝叶的挥发性成分,通过气相色谱质谱联用技术测定挥发性物质组成。结果结合质谱和保留指数分别从花和枝叶中鉴定出23和18种化合物,占各自化合物总量的85.73%和64.58%。四季桂花的香气主要由牻牛儿酸乙酯、反式-香叶醇、香叶酸甲酯、反式-柠檬醛、顺式-柠檬醛、茶香螺烷及壬醛组成;四季桂枝叶的鉴定的挥发性物质主要由酯类、醛类和碳氢化合物组成,主要释放乙酸叶醇酯、甲氧基乙酸-2-十三烷基酯、4,11-二甲基-十四烷、苯乙醛和苯乙醇。结论固相微萃取与气质联用技术便捷实用,适用于测定桂花和枝叶挥发性物质,从而为桂花资源开发利用、保护、及园林配置提供一定的理论依据。

ObjectiveTo characterize the volatiles in picked flowers and leaves ofOsmanthus fragransvar. semperflorens, and to understand the volatile composition and content in them.Methods Headspace-solid-phase microextraction and gas chromatography- mass spectroscopy (GC-MS) were used to characterize the volatiles in picked flowers and leaves ofOsmanthus fragrans var.semperflorens.Results A total of 23 components in the flowers and 18 in the leaves were identified by matching EI mass spectra and retention indices with the literature data, accounting for 85.73% and 64.58% total content of volatiles in flowers and leaves, respectively. Briefly, ethyl geranate, trans-geraniol, methyl geranate, trans-citral, 2,6,10,10-tetramethyl-1-oxa-spiro[4.5] dec-6-ene, cis-citral and nonanal were the most common volatiles in tested flowers. The major odors found in the leaves were cis-3-hexenyl acetate, 4,11-dimethyl- tetradecane, Methoxyacetic acid, 2-tridecyl ester, benzeneacetaldehyde and phenylethyl alcohol.Conclusion The method of HS-SPME/GC-MS is practical and suitable for the analysis of the volatile of picked flowers and leaves ofOsmanthus fragransvar.semperflorens, so as to facilitate the devel-opment ofOsmanthus scultivars to meet requirements of the food and fragrance industries.